| Pollen—embryogenesis and chromosomal variability in anther culture of Brassica hirta Moench （Sinapis alba L） |
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| 作者姓名： | BAJAJYPS DMOHAPTRA |
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| 作者单位： | [1]A－137NewFriendsColony,NewDelhi110065,India [2]NARP，R.R.S,Ranital,Balasore,Orissa756111,India |
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| 摘 要： | The anther cultures of Brassica hirta underwent pollenembryogenesis and callusing,which showed a wide range of chromosome numbers varying from 9 (n=12) to a highly polyploid.For embryogenesis,pretreatment of floral buds in 0.4 M sucrose solution for 72 hrs at 4℃ was superior to freshly cultured anthers.Culture temperature of 30℃ for 14 days before maintenance of cultures at 25℃ was significantly beneficial for embryo yield in comparison to cultures continuously incubated at 25℃.Dark treatment during culture was more effective for pollen-embryo yield.
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| 关 键 词： | 油料作物 白芥 花粉－胚胎发生 染色体变异性 |
Pollen-embryogenesis and chromosomal variability in anther culture of Brassica hirta Moench (Sinapis alba L) |
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| BAJAJYPS DMOHAPTRA.Pollen-embryogenesis and chromosomal variability in anther culture of Brassica hirta Moench (Sinapis alba L)[J].Cell Research,1990,1(2):191-196. |
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| Authors: | BAJAJ YPS AND D MOHAPTRA
A- New Friends Oolony New Delhi India
NARP RRS Ranital Balasore Orissa India |
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| Institution: | BAJAJ YPS AND D MOHAPTRA
A-137 New Friends Oolony,New Delhi 110065,India
NARP,R.R.S,Ranital,Balasore,Orissa 756111,India |
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| Abstract: | |
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| Keywords: | Pollen-embryogenesis anther culture Brassia hirta chromosomal variabiliiy |
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