| Expression and localization of VCX／Y proteins and their possible in-volvement in regulation of ribosome assembly during spermatogenesis |
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| 作者姓名： | Zou SW Zhang JC Zhang XD Miao SY Zong SD Sheng Q Wang LF |
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| 作者单位： | [1]NationalLaboratoryofMedicalMolecularBiology,InstituteofBasicMedicalSciences,ChineseAcademyofMedicalSciences,PekingUnionMedicalCollege,5DongDan3-tiao,Beijing100005,China [2]NationalResearchInstituteforFamilyPlaning,WHOCollaborationCenterforResearchinHumanRe-production,12DaHuiSi,Beijing100081,China |
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| 摘 要： | Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromo-somes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST)fusion protein. With the purified fusion protein as antigen, the anti-GST-VCY antibody was generated andthe localization of VCY protein in human testis was determined by immunohistochemistry. In the testisseminiferous epithelium, VCY proteins were highly expressed in nuclei of germ cells. Using propidium io-dide staining and green fluorescent protein (GFP) tag technologies, VCY and VCX-8r proteins were mainlylocalized in the nucleoli of COS7 cells. In addition, the colocalization for VCY and VCX-8r in COS7 cellswas also observed. With VCY cDNA as bait, a cDNA fragment of acidic ribosomal protein PO was obtainedusing yeast two-hybrid system. All the information above indicates that VCX/Y protein family might beinvolved in the regulation of ribosome assembly during spermatogenesis.
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| 关 键 词： | 精子发生 核糖体装配 定位 调控 VCX／Y蛋白 |
Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis |
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| Zou SW,Zhang JC,Zhang XD,Miao SY,Zong SD,Sheng Q,Wang LF.Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis[J].Cell Research,2003,13(3):171-177. |
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| Authors: | Zou Sheng Wei Zhang Jian Chao Zhang Xiao Dong Miao Shi Ying Zong Shu Dong Sheng Qi Wang Lin Fang |
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| Institution: | National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan 3-tiao, Beijing 100005, China. |
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| Abstract: | Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromosomes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST) fusion protein. With the purified fusion protein as antigen, the anti-GST-VCY antibody was generated and the localization of VCY protein in human testis was determined by immunohistochemistry. In the testis seminiferous epithelium. VCY proteins were highly expressed in nuclei of germ cells. Using propidium iodide staining and green fluorescent protein (GFP) tag technologies, VCY and VCX-8r proteins were mainly localized in the uucleoli of COST cells. In addition, the colocalization for VCY and VCX-8r in COS7 cells was also observed. With VCY cDNA as bait, a cDNA fragment of acidic ribosomal protein PO was obtained using yeast two-hybrid system. All the information above indicates that VCX/Y protein family might be involved in the regulation of ribosome assembly (hiring spermatogenesis. |
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| Keywords: | VCX/Y protein spermatogenesis nucleoli colocnlization |
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