| The culture and establishment of embryonic germ （EG） cell lines from Chinese mini swine |
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| 作者姓名： | Tsung HC Du ZW Rui R Li XL Bao LP Wu J Bao SM Yao Z |
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| 作者单位： | [1]LaboratoryofEmbryonicStemCell,InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologcalSciences,ChineseAcademyofSciences,Shanqhai200031.China [2]ShanghaiLaboratoryAnimalCenter,ChineseAcademyofScienceslShanghai200233,China |
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| 摘 要： | As a part of a basic research project on Xeno-transplantion, we have been engaged in the derivation of embryonic stem cell lines from Chinese mini swine. Here, we reported for the first time the establishment of two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 anda 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, marker characterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentially useful for further basic studies.
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| 关 键 词： | EG细胞 中国小猪 胚胎 体外试验 |
The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine |
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| Tsung HC,Du ZW,Rui R,Li XL,Bao LP,Wu J,Bao SM,Yao Z.The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine[J].Cell Research,2003,13(3):195-202. |
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| Authors: | Tsung Hsiao Chien Du Zhong Wei Rui Rong Li Xiu Lan Bao Lin Ping Wu Jun Bao Shi Min Yao Zhen |
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| Institution: | Laboratory of Embryonic Stem Cell, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. escg@sunm.shcnc.ac.cn |
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| Abstract: | As a part of a basic research project on Xeno-transplantion. we have been engaged in the derivation of embryonic stein cell lines from Chinese mini swine. Here, we reported for the first time the establishment of two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 and a 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, marker characterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentially useful for further basic studies. |
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| Keywords: | primordial germ cells EG cells porcine Chinese mini swine |
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