| Using a non—radioisotopic,quantitative TRAP—based method detecting telomerase activities in human hepatoma cellos |
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| 作者姓名： | Zhang RG Wang XW Yuan JH Guo LX Xie H |
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| 作者单位： | [1]ShanghaiInstituteofCellBiology,ChineseAcademyofSciences,Shanghai200031,China [2]ShanghaiInstituteofCellB,ChineseAcademyofSciences,Shanghai200031,China |
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| 摘 要： | A non-radioisotopic,quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope.Comparing with conventional radioisotope based method,it was better in reproducibility and accuracy.Using this method,we found telomerase activities were absent in normal human liver cells,while detected in all of four human hepatoma cell lines (BEL-7404,SMMC-7721,QGY-07903 and HCCM) without significant differences.
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| 关 键 词： | 人肝癌细胞 瑞粒酶活性 检测 非放射性同位素 定量 TRAP |
Using a non-radioisotopic, quantitative TRAP-based method detecting telomerase activities in human hepatoma cells |
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| Zhang RG,Wang XW,Yuan JH,Guo LX,Xie H.Using a non-radioisotopic, quantitative TRAP-based method detecting telomerase activities in human hepatoma cells[J].Cell Research,2000,10(1):71-77. |
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| Authors: | Zhang R G Wang X W Yuan J H Guo L X Xie H |
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| Institution: | Shanghai Institute of Cell Biology, Chinese Academy of Sciences. |
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| Abstract: | A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in all of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences. |
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