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| 病原性丝状真菌的菌种保藏方法 | |
| 引用本文： | 吴婧,李东明,G. S.deHoog,姚一建.病原性丝状真菌的菌种保藏方法[J].中国真菌学杂志,2011,6(5):305-307. |
| 作者姓名： | 吴婧 李东明 G. S.deHoog 姚一建 |
| 作者单位： | 1. 中国科学院微生物研究所真菌地衣系统学重点实验室,北京 100101;北京大学第三医院,北京 100191 2. 北京大学第三医院,北京,100191 3. Centraalbureau voor Schimmelcultures, Utrecht, Netherlands 4. 中国科学院微生物研究所真菌地衣系统学重点实验室,北京,100101 |
| 摘 要： | 目的 探讨常见病原性丝状真菌的菌种保藏方法.方法将73株病原性丝状真菌经过纯化后,接种于马铃薯葡萄糖琼脂斜面分别在4℃和-80℃冷冻管保存,均用10％ (v/v)的丙三醇作为保护剂.结果经过1 a左右的保存,将菌株复活,发现4℃斜面保藏法菌株的存活率为100％,-80℃冷冻管保藏法菌株的存活率为98.6％,有些毛癣菌属... |
| 关 键 词： | 病原性真菌 菌种保藏 丝状真菌 |
Preservation of pathogenic fiflamentous fungi |
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| WU Jing,LI Dong-ming,G. S.deHoog,YAO Yi-jian.Preservation of pathogenic fiflamentous fungi[J].Chinese JOurnal of Mycology,2011,6(5):305-307. | |
| Authors: | WU Jing LI Dong-ming G SdeHoog YAO Yi-jian |
| Institution: | WU Jing1,2,LI Dong-ming2,G.S.de Hoog3,YAO Yi-jian1 (1.Key Laboratory of Systematic Mycology and Lichenology,Institute of Microbiology,Chinese Academy of Science,Peking 100101,China,2.Peking University Third Hospital,Peking 100191,3.Centraalbureau voor Schimmelcultures,Utrecht,Netherlands) |
| Abstract: | Objective To explore a practical and effective method for fingi preservation.Methods Seventy-three strains of pathogenic filamentous fungi were preserved on Potato dextrose agar slants at 4℃ and in freezing tubes at-80℃,overlaid with 10%（v/v） glycerol after purification.Results The survival ratioes were 98.6% at-80℃ and 100% at 4℃ after one-year preservation.Conclusions These two methods are simple,convenient,and valueble in preservation of pathogenic filamentous fungi. |
| Keywords: | pathogenic fungi culture collection filamentous fungi |
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