TLR2在红色毛癣菌感染中对角质形成细胞分泌γ-IFN和IL-8的影响

目的 观察红色毛癣菌刺激人角质形成细胞后γ-IFN及IL-8浓度的变化,以及TLR2对γ-IFN和IL-8分泌的影响.方法 用红色毛癣菌悬液分别刺激TLR2抗体处理前后的角质形成细胞,采用ELISA方法检测不同时间点细胞上清液中γ-IFN及IL-8的浓度,并设置阴性对照;比较TLR2抗体处理的后γ-IFN及IL-8浓度的变化.结果 红色毛癣菌刺激角质形成细胞后,γ-IFN及IL-8浓度明显升高(P＜0.05),4h即开始,至16 h达高峰;用TLR2抗体中和TLR2后,上清液中IL-8的浓度在2h、4h、8h、16h各时间点较中和前低,差异有统计学意义(P＜0.05);γ-IFN的浓度2h、4h、8h时间点较中和前低,差异有统计学意义(P＜0.05),而在16h时间点,上清液中γ-IFN的浓度与中和前比较略低,但差异没有统计学意义(P＞0.05).结论 红色毛癣菌刺激角质形成细胞后,可促进角质形成细胞分泌γ-IFN和IL-8;TLR2在角质形成细胞分泌γ-IFN和IL-8的过程中发挥重要的调节作用.

Effects of TLR2 on IL-8 and γ-IFN secretion of keratinocytes in Trichophyton rubrum infection

Objective Observe the change of γ-IFN and IL-8 secreted by keratinocytes after Trichophyton rubrum infection,and the effects of TLR2 on γ-IFN and IL-8 secretion.To explore the role of TLR2 in anti-dermatophytic infection.Methods Keratinocytes treated before and after anti-TLR2 monoantibody were stimulated by Trichophyton rubrum.The levels of γ-IFN and IL-8 in supernatant were determined with ELISA at different time points.Results The levels of γ-IFN and IL-8 secreted by kerainocytes were significantly increased after induced with Trichophyton rubrum(P<0.05).The level of γ-IFN reached(85.36±4.54) pg/mL at the point of 4h,and(445.58±13.99) pg/mL at 16 h;After treated with anti-TLR2 monoantibody,the levels of IL-8 were reduced at the points of 2 h,4 h,8 h and 16 h(P<0.05);and the levels of γ-IFN were reduced at the points of 2 h,4 h and 8 h(P<0.05),but had no statistically significant reduction at the point of 16 h(P>0.05).Conclusion After stimulated keratinocytes,Trichophyton rubrum can promote its secretion of γ-IFN and IL-8;TLR2 play an important role onγ-IFN and IL-8 secretion of keratinocytes.