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| 应用RAPD技术对我国不同地域红色毛癣菌分离株的遗传多样性研究 | |
| 引用本文： | 鲁莎,鲁长明,吴绍熙,李春阳,沈永年,李希清.应用RAPD技术对我国不同地域红色毛癣菌分离株的遗传多样性研究[J].中国真菌学杂志,2010,5(4):206-209,229. |
| 作者姓名： | 鲁莎 鲁长明 吴绍熙 李春阳 沈永年 李希清 |
| 作者单位： | 1. 中山大学孙逸仙纪念医院皮肤科,广州,510120 2. 中国科学院皮肤病研究所,南京,210042 3. 山东大学齐鲁医院皮肤科,济南,250062 |
| 摘 要： | 目的探讨我国不同地域红色毛癣菌分离株的遗传多样性。方法采用随机扩增DNA多态性(RAPD)方法对来源于我国不同地域(江苏南京,山东济南,广东广州)的32株红色毛癣菌临床分离株进行DNA多态性分析。结果红色毛癣菌种内差异明显,根据遗传相似性分成三大聚类群,与地域差异及取材部位无明显相关性,而与表型具有一定相关性。结论随机扩增DNA多态性方法可用于红色毛癣菌的DNA分型,其DNA带型具有一定的遗传变异性,与菌株表型有一定关系,与地域差异、侵犯部位无明显相关性。 |
| 关 键 词： | 红色毛癣菌 遗传多样性 随机扩增DNA多态性方法 |
Genetic diversity of Trichophyton rubrum from different regions of China by random amplified polymorphic DNA |
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| LU Sha,LU Chang-ming,WU Shao-xi,LI Chun-yang,SHEN Yong-nian,LI Xi-qing.Genetic diversity of Trichophyton rubrum from different regions of China by random amplified polymorphic DNA[J].Chinese JOurnal of Mycology,2010,5(4):206-209,229. | |
| Authors: | LU Sha LU Chang-ming WU Shao-xi LI Chun-yang SHEN Yong-nian LI Xi-qing |
| Institution: | 1.Department of Dermatology,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China;2.Department of Fungal Skin Disease,Institute of Dermatology,CAMS and PUMC,Nanjing 210042,China;3.Department of Dermatology,Qilu Hospital of Shandong University,Jinan 250012,China) |
| Abstract: | Objective To study the genetic diversity of T.rubrum from different geographical origins of China（Nanking,Jinan,Guangzhou）.Methods Totally 32 clinical isolates of T.rubrum were collected and analyzed by random amplified polymorphic DNA（RAPD）analysis.Results Subsequent analysis of the gel profiles by computerized cluster-derived dendrograms revealed that significant intraspecific differences were relevant to phenotype,but not the region or isolated sources which divided Trichophyton rubrum into 3 cluster groups.Conclusions RAPD assay is useful for molecular typing of T.rubrum. |
| Keywords: | Trichophyton rubrum genetic diversity random amplified polymorphic DNA method |
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