PCR指纹图谱分析在念珠菌性阴道炎、龟头炎流行病学研究中的应用

目的 建立一种鉴别念珠菌性阴道炎和龟头炎传染、复发及再感染的分子生物学方法.方法 对实验菌株进行微卫星PCR扩增,对所获得的PCR指纹图谱多态性进行分析,比较两次感染或夫妻同时感染的病原菌株的亲缘性关系.结果 22株实验菌株中,8株来自4例患者前后两次感染的两株,图谱相似系数大于0.8,可以认为是复发,3对夫妻间感染的6株菌株相似系数大于0.8,可以认定是性传染,其余8株菌的相似系数小于0.8,可以认为是再感染.结论 微卫星PCR指纹图谱能在一定程度上说明念珠菌阴道炎、龟头炎发病及复发的原因,在鉴别感染是由传染、复发还是再感染所致时中有一定的参考价值,可为临床治疗提供参考.

Applications of PCR fingerprinting in the study of epidemiology of Candidal vulvovaginitis and balanitis

Objective To establish a molecular biological method of identifying infection,recrudescence or reinfection of candidal vulvovaginitis and balanitis.Methods PCR was used to amplify the microsatellite region of the two pathogens from patients infected twice or couples infected simultaneously.The products were analyzed according to PCR fingerprinting polymorphism to determine the relationship between the two strains.Results Of the 22 tested strains,8 strains from 4 individual patients got a similarity module above 0.8,which was considered recrudescence;6 strains from 3 pairs of couple also displayed a similarity module above 0.8,which was regarded as a result of sexual transmission.The rest got a similarity module below 0.8,which could be considered reinfection.Conclusion To some extent,the method of microsatellite primed PCR fingerprint spectrum could identify infection,recrudescence or reinfection,the result of which could be suggestive in clinical treatment.