Eradication of melanoma in vitro and in vivo via targeting with a Killer-Red-containing telomerase-dependent adenovirus |
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| Authors: | Kiyoto Takehara Shuya Yano Hiroshi Tazawa Hiroyuki Kishimoto Nobuhiro Narii Hiroyuki Mizuguchi |
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| Institution: | 1. AntiCancer, Inc., San Diego, CA, USA;2. Department of Surgery, University of California San Diego, San Diego, CA, USA;3. Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;4. Center for Innovative Clinical Medicine, Okayama University Hospital, Okayama, Japan;5. Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;6. Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan |
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| Abstract: | Melanoma is a highly recalcitrant cancer and transformative therapy is necessary for the cure of this disease. We recently developed a telomerase-dependent adenovirus containing the fluorescent protein Killer-Red. In the present report, we first determined the efficacy of Killer-Red adenovirus combined with laser irradiation on human melanoma cell lines in vitro. Cell viability of human melanoma cells was reduced in a dose-dependent and irradiation-time-dependent manner. We used an intradermal xenografted melanoma model in nude mice to determine efficacy of the Killer-Red adenovirus. Intratumoral injection of Killer-Red adenovirus, combined with laser irradiation, eradicated the melanoma indicating the potential of a new paradigm of cancer therapy. |
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| Keywords: | adenovirus killer-red melanoma nude mouse photodynamic therapy telomerase |
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