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| 原生质体激光诱变选育少根根霉脂肪酶高产菌株 | |
| 引用本文： | 冀颐之,李政,谭天伟,杜连祥,林强.原生质体激光诱变选育少根根霉脂肪酶高产菌株[J].工业微生物,2011,41(3):38-43. |
| 作者姓名： | 冀颐之 李政 谭天伟 杜连祥 林强 |
| 作者单位： | 1. 天津科技大学生物工程学院,天津市工业微生物重点实验室,天津,300222;北京联合大学生物化学工程学院,北京,100023 2. 天津工业大学纺织学院,天津,300160 3. 北京化工大学教育部生物炼制工程中心,北京,100029 4. 天津科技大学生物工程学院,天津市工业微生物重点实验室,天津,300222 5. 北京联合大学生物化学工程学院,北京,100023 |
| 基金项目： | 国家"863"项目，国家"十五"攻关项目，国家自然科学基金项目，中石化项目 |
| 摘 要： | 对少根根霉BUCT-11原生质体制备、再生条件及激光诱变育种进行了研究.结果显示,少根根霉BUCT-11原生质体形成及再生最佳条件为:菌龄24 h,混合酶由27 mg/mL的蜗牛酶和53 mg/mL的纤维素酶组成,酶解时间1.5 h,酶解温度30 ℃,渗透压稳定剂为0.6 mol/L NH4Cl、0.02 mol/L ... |
| 关 键 词： | 脂肪酶 少根根霉 激光 原生质体 诱变选育 |
Breeding of Rhizopus arrhizus lipase producing strain by laser induced protoplast mutagenesis |
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| JI Yi-zhi,LI Zheng,TAN Tian-wei,DU Lian-xiang,LIN Qiang.Breeding of Rhizopus arrhizus lipase producing strain by laser induced protoplast mutagenesis[J].Industrial Microbiology,2011,41(3):38-43. | |
| Authors: | JI Yi-zhi LI Zheng TAN Tian-wei DU Lian-xiang LIN Qiang |
| Institution: | 1. Tianjin Key Lab of Industrial Microbiology , School of Bioengineering , Tianjin University of Science and Technology Tianjin 300222, China; 2. Biochemical Engineering College of Beijing Union University, Beijing 100023, China; 3. School of Textiles, Tianjin Polytechnic University, Tianjin 300160, China; 4. Biorefinery Engineering Center of Ministry of Education, Beijing University of Chemical and Teehnoiogy, Beijing 100029, China) |
| Abstract: | The conditions of protoplast formation, regeneration and laser mutagenesis of Rhizopus arrhizus BUCT-11 strain were investegated. As a result, the optimum conditions for protoplast preparation and regeneration of Rhizopus arrhizus BUCT-11 strain were as follows： 24 h of mycelium growth time, compound enzyme containing 27 mg/mL of snailase and 53 mg/mL of fibrin enzyme, 1.5 h hydrolysis, hydrolysis temperature at 30℃, applying 0.6 mol/L NH4 Cl, 0.02 mol/L MgSO4·7H2O, 0.4 mol/L CaC12 phosphate buffer for osmotic stabilizer. The He-Ne laser was irradiated on protoplasts of strain BUCT-11. The high-yielding lipase mutant numbered Y007 with good genetic stability was obtained, and its lipase yield in shaking flask scale was up to 151 U/mL, which was 41% higher than that of the parent strain. |
| Keywords: | lipase Rhizopus arrhizus laser protoplast mutation breeding |
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