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| 适冷溶菌酶产生菌的分子鉴定及其酶学性质的研究 | |
| 引用本文： | 王全富,侯艳华,沈继红,林学政,闫培生,李宁.适冷溶菌酶产生菌的分子鉴定及其酶学性质的研究[J].工业微生物,2011,41(3):50-53. |
| 作者姓名： | 王全富 侯艳华 沈继红 林学政 闫培生 李宁 |
| 作者单位： | 1. 哈尔滨工业大学化工学院,黑龙江哈尔滨,150010;哈尔滨工业大学威海校区海洋学院,山东威海,264209 2. 哈尔滨工业大学威海校区海洋学院,山东威海,264209 3. 国家海洋局第一研究所海洋生物活性物质重点实验室,山东青岛,266061 4. 哈尔滨工业大学化工学院,黑龙江哈尔滨,150010 |
| 基金项目： | 国家863计划资助项目，中国博士后科学基金，黑龙江省博士基金;山东省自然科学基金，威海市科技攻关项目，哈尔滨工业大学优秀创新团队支持计划和科研创新基金 |
| 摘 要： | 采用比浊法对本试验室保存的126株南极微生物产适冷溶菌酶进行测定,发现7株菌产溶菌酶.并对其中的菌株NJ147进行ITS-5.8 S rDNA基因序列的同源性和系统发育分析,结果表明:菌株NJ147属于Debaryomyces hansenii属.该菌株所分泌溶菌酶的最适作用温度为30 ℃,在0 ℃时酶活性是最高活性的... |
| 关 键 词： | 适冷溶菌酶 南极微生物 ITS-5.8 S rDNA Debaryomyces hanseni |
Molecular identification of cold-active lysozyme-producing strain and enzyme characteristics |
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| WANG Quan-fu,HOU Yan-hua,SHEN Ji-hong,LIN Xue-zheng,YAN Pei-sheng,LI Ning.Molecular identification of cold-active lysozyme-producing strain and enzyme characteristics[J].Industrial Microbiology,2011,41(3):50-53. | |
| Authors: | WANG Quan-fu HOU Yan-hua SHEN Ji-hong LIN Xue-zheng YAN Pei-sheng LI Ning |
| Institution: | 1. Department of Applied Chemistry, Harbin Institute of Technology, Heilongjiang Harbin, 150001; 2. School of Ocean, Harbin Institute of Technology at Weihai, Shandong Weihai ,264209) 3. Key Laboratory of Marine Bio-active Substances, First Institute of Oceanorgarphy, State Oceanic Administration, Shandong Qingdao 266061 ) |
| Abstract: | Seven stains producing cold-active lysozyme were screned from 126 strains of antarctic microorganisms using nephelometry assay. The ITS-5.8S rDNA gene sequences homology and phylogenetic analysis showed that the strain NJ147 belonged to the genus Debaryomyces hanseniis. The lysozyme characteristics were studied. The optimum lysozyme activity was observed at 30℃ and about 32.6 % of the optimum activity was still detectable at 0℃. It was thermal lability, the enzyme ti was at 40℃ for 30 min. The optimum enzyme activity was observed at pH 6. This lysozyme showed typical features of a cold-adapted enzyme. It had broad-spectrum against 6 kinds of strains and had potential in industry. |
| Keywords: | ITS-5 8 S rDNA Debaryomyces hanseni |
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