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| 肠膜明串珠菌葡聚糖蔗糖酶基因的克隆与表达 | |
| 引用本文： | 农万廷,韦宇拓,黄日波.肠膜明串珠菌葡聚糖蔗糖酶基因的克隆与表达[J].工业微生物,2007,37(3):29-32. |
| 作者姓名： | 农万廷 韦宇拓 黄日波 |
| 作者单位： | 1. 广西大学广西亚热带生物资源保护与利用重点实验室,南宁,530005;广西南宁师范高等专科学校化生系,龙州,532400 2. 广西大学广西亚热带生物资源保护与利用重点实验室,南宁,530005 3. 广西大学广西亚热带生物资源保护与利用重点实验室,南宁,530005;广西科学院广西工业生物技术研究中心,南宁,530004 |
| 基金项目： | 广西大学校科研和教改项目 |
| 摘 要： | 利用PCR方法从肠膜明串珠菌葡聚糖亚种(Leuconostoc mesenteroides subsp dextranicum)基因组DNA中扩增出了葡聚糖蔗糖酶基因dsrD并将其连接到表达栽体pET-30(a),得到重组质粒pET-30-dsrD,将重组质粒转化到大肠杆菌菌株Rosetta中,重组菌株SDS-PAGE结果显示有明显的170kD特异蛋白条带出现.经测定酶活力达1.2U/mL,约是原始菌株的30倍. |
| 关 键 词： | 肠膜明串珠菌 葡聚糖蔗糖酶基因 克隆 表达 |
Cloning and expression of glucansucrase from L.mesenteroides in Escherichia coli |
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| NONG Wan-ring,WEI Yu-tuo,HUANG Ri-bo.Cloning and expression of glucansucrase from L.mesenteroides in Escherichia coli[J].Industrial Microbiology,2007,37(3):29-32. | |
| Authors: | NONG Wan-ring WEI Yu-tuo HUANG Ri-bo |
| Institution: | 1. Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning 530005, China 2. GuangXi Nanning Teachers College, Longzhou 532400, China; 3. Guagxi Academy of Sciences, Nanning 530004 |
| Abstract: | The genes dsrD encoding glucansucrase of Leuconostoc mesenteroides subsp.dextranicum were amplified by PCR.The recombinant plasmid pET-30-dsrD was constructed by inserting genes dsrD into expression vector pET-30 and then transformed into E.coli Rosetta(DE3).The recombinant strain was induced by IPTG to express dsrD.Glucansucrase was analysed and conformed by sodium dodecyl sulfa-polyacrylamide gel electrophoresis.The deduced products of the dsrD gene with calculated molecular masses of 170KD,revealed high activity.By comparison,no activity appeared in hosted E.coli Rosetta(DE3). |
| Keywords: | Leuconostoc mesenteroide glucansucrase gene cloing expression |
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