| 高浓度丁醇浸泡及N＋束注入诱变双重作用筛选丁醇高产菌 |
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| 引用本文： | 谭秀花,余晓斌,常江波.高浓度丁醇浸泡及N＋束注入诱变双重作用筛选丁醇高产菌[J].工业微生物,2011,41(4):16-20. |
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| 作者姓名： | 谭秀花 余晓斌 常江波 |
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| 作者单位： | 江南大学生物工程学院,工业生物技术教育部重点实验室,江苏,无锡,214122 |
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| 基金项目： | 教育部长江学者和创新团队发展计划资助项目，江苏高校优势学科建设工程资助项目 |
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| 摘 要： | 通过高浓度丁醇浸泡处理丙酮丁醇梭菌（Clostridiumacetobutylicum）CL-2，筛选得到一株丁醇耐受能力提高并溶剂产量增加的菌株BR30—2，丁醇产量达11．77g/L，比CL-2提高了16．65％。以BR30—2作为出发菌株，进行N＋束注入诱变，筛选得到高产菌株BH．9，丁醇产量达14．5g/L，总溶剂为23．14g／L。在BH-9发酵过程中添加0．1％丁酸钠，丁醇产量达到16．59g/L，丁醇比例提高至67．38％。
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| 关 键 词： | 高浓度丁醇浸泡 N＋束注入 丁醇 |
Screening of a high butanol-yielding strain by high concentration butanol steeping and N+ beam implantation |
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| TAN Xiu-hua,Yu Xiao-bin,CHANG Jiang-bo.Screening of a high butanol-yielding strain by high concentration butanol steeping and N+ beam implantation[J].Industrial Microbiology,2011,41(4):16-20. |
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| Authors: | TAN Xiu-hua Yu Xiao-bin CHANG Jiang-bo |
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| Institution: | (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China) |
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| Abstract: | A higher butanol tolerance and butanol-yielding strain BR30-2 was screened from Clostridium acetobutylicum CL-2 after treated with high concentration butanol steeping and its output of butanol reached 11.77 g/L, increased by 16.65% than that of CL-2. Using BR30-2 as the starting strain for further mutation of N＋ beam implantation, a high butanol-yielding strain BH-9 was obtained, its butanol yield was as high as 14.51 g/L and the solvent yield was 23.14 g/L. After adding 0.1% sodium butyrate into the media during the fermentation by mutant BH-9, the output of butanol reached 16.59 g/L and the butanol ratio was up to 67.38 %. |
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| Keywords: | high concentration butanol steeping N＋ beam implantation butanol |
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