| 产抑菌物质乳杆菌的筛选及性质的研究 |
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| 引用本文： | 杨颖,陈卫,田丰伟,张灏,汤坚.产抑菌物质乳杆菌的筛选及性质的研究[J].工业微生物,2006,36(3):13-17. |
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| 作者姓名： | 杨颖 陈卫 田丰伟 张灏 汤坚 |
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| 作者单位： | 江南大学,食品科学与安全教育部重点实验室,江苏,无锡,214036 |
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| 摘 要： | 从健康成年人的口腔中筛选出一株产抑菌物质的菌株HO-69,经API系统鉴定为植物乳杆菌。排除酸性末端产物与过氧化氢的影响后,HO-69的发酵上清液对变形链球菌等革兰氏阳性菌、大肠杆菌等革兰氏阴性菌具有抑制作用。抑菌物质经初步提取,推测其分子量在10kDa以下,胰蛋白酶处理后抑菌活性部分损失,100℃水浴加热20min依然保持较高的抑菌活力,显示活性的pH范围是3.0~7.0,活力随pH的降低而增强。
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| 关 键 词： | 植物乳杆菌 抑菌物质 筛选 性质 鉴定 |
Antibacterial substance producing Lactobacillus strain: selection and characterization |
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| YANG Ying,CHEN Wei,TIAN Feng-wei,ZHANG Hao,TANG Jian.Antibacterial substance producing Lactobacillus strain: selection and characterization[J].Industrial Microbiology,2006,36(3):13-17. |
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| Authors: | YANG Ying CHEN Wei TIAN Feng-wei ZHANG Hao TANG Jian |
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| Institution: | Key Lab of Food Science and Safety, Ministry of Education , Southern Yangtze University , Wuxi 214036, China |
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| Abstract: | HO-69,a strain producing antibacterial substance,was isolated from healthy oral cavity,and was identified by API system as Lactobacillus plantarum.The supernatant of HO-69 exhibited inhibitory activty against gram-positive and gram-negative bacteria including Streptococcus mutants and Escherichia coli,eliminated the effects of organic acids and hydrogen peroxide.The antibacterial substance had a low molecular weight less than 10kDa.It was sensitive to pepsin,and was heat stable.It was active within pH range from 3 to 7,and it showed higher activity at lower pH. |
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| Keywords: | Lactobacillus plantarum antibacterial substance screen identification characterization |
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