| 一株生淀粉酶产生菌的分离鉴定及酶学性质研究 |
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| 引用本文： | 李彧娜,石贵阳,王武,王正祥.一株生淀粉酶产生菌的分离鉴定及酶学性质研究[J].工业微生物,2010,40(1):64-68. |
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| 作者姓名： | 李彧娜 石贵阳 王武 王正祥 |
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| 作者单位： | 江南大学生物工程学院工业生物技术教育部重点实验室,无锡,214122;江南大学生物工程学院生物资源与生物能源研究中心,无锡,214122 |
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| 基金项目： | 国家高技术研究发展计划"863"项目 |
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| 摘 要： | 采用苔酚蓝-生淀粉法从贵州遵义百年磨坊的磨盘下的土样中筛选得到一株具有生淀粉降解能力的丝状真菌,经形态学鉴定和ITS核苷酸序列比对确定该菌为Rhizopus microsporusvar.chinensis。本文首次报道了由Rhizopus microsporus var.chinensis产生的生淀粉酶。通过酶学性质研究发现,该菌株所产生淀粉酶拥有较好的温度耐受性和较宽的pH适用范围,具有一定的工业应用价值。
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| 关 键 词： | 生淀粉酶 分离鉴定 Rhizopus microsporus var．chinensis 酶学性质 |
Screening and identification of a fungus producing raw-starch amylase with new enzymatic properties |
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| LI Yu-na,SHI Gui-yang,WU Wang,WANG Zheng-xiang.Screening and identification of a fungus producing raw-starch amylase with new enzymatic properties[J].Industrial Microbiology,2010,40(1):64-68. |
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| Authors: | LI Yu-na SHI Gui-yang WU Wang WANG Zheng-xiang |
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| Institution: | 1. School of Biotechnology and Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University, Wuxi 214122,China; 2. Research Center of Bio-resource and Bio-energy, School of Biotechnology, Jiangnan University, Wuxi 214122, China) |
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| Abstract: | A newly isolated producer of raw starch digesting amylase, Rhizopus microsporus var. chinensis was screened and identified by morphological characteristics and molecular phylogenetic analysis. For the first time, raw starch digesting amylase produced by fungus of Rhizopus microsporus var. chinensis was reported. Further studies found that the raw starch digesting amylase secreted by this strain performed a favorable thermostabilization and broad pH available range, which was valuable for industrial applications. |
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| Keywords: | Rhizopus microsporus var chinensis raw starch digesting amylase screening and identification Rhizopus microsporus var chinensis enzymatic properties |
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