中间产物对玫瑰茄培养细胞合成花青苷的影响

用Ｂ５培养基悬浮培养产色素的玫瑰茄培养细胞，培养１３天时，花青苷产量最高，为０．２５ｇ／Ｌ。培养基中添加终浓度为１０＾－６ｍｏｌ／Ｌ的外源Ｌ－Ｐｈｅ能够显著地增加产色素细胞花青苷的积累量。浓度为１０＾－７ｍｏｌ／Ｌ的槲皮素，可使悬浮培养的玫瑰茄细胞花青苷产量提高１．３倍，无论是Ｌ－Ｐｈｅ还是槲皮素均不能启动不产色素的细胞系产花青苷。

Effects of Intermediates on Accumulation of Anthocyanin in Roselle(Hibiscus sabdariffa L.)Cell Suspension

When pigmented cells of roselle (Hibiscus sabdariffa L.) Suspension culture grew in B5 medium for thirteen days, best accumlation of anthocyanin as high as 0.25 g/L was obtained. The anthocyanin yield of pigmented suspension cultured cells was significantly increased if 10-6 mol/L of exogenous L-Phe was added into B5 medium. When 10-7 mol/L of quercetin was supplied into the medium, the pigment yield was increased 1.3 fold than that of the control. Either L-Phe or quercetin feeding did not initiate anthocyanin accumulation in cell lines which were not anthocyanin accumulation.