| D-苯丙氨酸产生菌的诱变育种 |
| |
| 引用本文： | 李环,韦萍,顾海红,欧阳平凯.D-苯丙氨酸产生菌的诱变育种[J].工业微生物,2003,33(3):6-10. |
| |
| 作者姓名： | 李环 韦萍 顾海红 欧阳平凯 |
| |
| 作者单位： | 南京工业大学制药与生命科学学院,南京,210009 |
| |
| 基金项目： | 国家十五攻关项目 2 0 0 1BA70 8B0 2 -0 6 |
| |
| 摘 要： | 先后使用紫外与5-FU复合处理及Nd：YAG倍频脉冲激光辐照等方法对D-苯丙氨酸产生菌Pseudomonas putida JS-01进行诱变，筛选到一株稳定高产的D-苯丙氨酸产生菌1003。对底物5-苄基海因的转化率由55．3％上升到85．5％，提高率为54．6％。较高的底物浓度亦能保持较高的转化能力。
|
| 关 键 词： | D—苯丙氨酸 产生菌 诱变 育种 紫外线 激光 |
Improvement of D-phenylalanine producing strains by mutation |
| |
| LI Huan,WEI Ping,GU Hai hong,OUYang Ping kai.Improvement of D-phenylalanine producing strains by mutation[J].Industrial Microbiology,2003,33(3):6-10. |
| |
| Authors: | LI Huan WEI Ping GU Hai hong OUYang Ping kai |
| |
| Abstract: | After the D Phenylalanine producing strain Pseudomonas putida JS 01 was mutagenized by ultraviolet ray+5 fluorouracil and Nd:YAG laser, a high yielding mutant 1003 was obtained. 85.5% substrate could be transformed to the product, which was 54.6% higher than that of the original strain. The transformation could keep steady at a higher substrate concentration. |
| |
| Keywords: | D phenylalanine ultraviolet ray laser mutation breeding |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
