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产核酸和乳糖酶的热带假丝酵母QZN0209培养条件研究
引用本文:周震,邱蔚然,闫蓬勃.产核酸和乳糖酶的热带假丝酵母QZN0209培养条件研究[J].工业微生物,2002,32(3):28-32.
作者姓名:周震  邱蔚然  闫蓬勃
作者单位:1. 华东理工大学生物反应器工程国家重点实验室,上海,200237
2. 上海秋之友生物科技有限公司,上海,200090
摘    要:经筛选得到一株具有一定核酸含量和乳糖酶活力的热带假丝酵母(Candida tropicalis)QZN0209,以综合利用酵母为目的,通过着重优化该菌株产核酸的培养条件。将其摇瓶产核酸水平从4.16%提高至13.94%;同时改变乳糖诱导方法将其乳糖酶活力提高约4倍,达到10.56ONPG单位/g湿菌体。

关 键 词:核酸  乳糖酶  热带假丝酵母  QZN0209  培养条件

Studies on culture conditions of nucleic acid-producing and lactase-producing Candida tropicalis QZN0209
ZHOU Zhen,QIU Wei-ran,YAN Peng-bo.Studies on culture conditions of nucleic acid-producing and lactase-producing Candida tropicalis QZN0209[J].Industrial Microbiology,2002,32(3):28-32.
Authors:ZHOU Zhen  QIU Wei-ran  YAN Peng-bo
Abstract:The fermentation conditions of Candida tropicalis QZN0209 producing nucleic acid and lactase were studied. The yield of nucleic acid was increased from 4.16% to 13.94 % by optimization of its fermentative medium and main parameters such as inoculation and feeding. And the lactase productivity was improved by adding lactose after logarithm phase in fermentation to10.56 ONPG units/g(wt.)
Keywords:Candida tropicalis  ribonucleic acid  lactase
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