| pH控制下绿色木霉（Trichoderma viride）流加发酵生产纤维素酶 |
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| 引用本文： | 耿冰,郭美锦,张嗣良,王永红,储炬,庄英萍.pH控制下绿色木霉（Trichoderma viride）流加发酵生产纤维素酶[J].工业微生物,2009,39(4):21-26. |
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| 作者姓名： | 耿冰 郭美锦 张嗣良 王永红 储炬 庄英萍 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 摘 要： | 绿色木霉（Trichodermaviride）在pH控制发酵条件下，采用流加葡萄糖发酵策略，可显著提高综合滤纸酶活力（FPA）和内切酶（endo—β—1，4-glucanase，EG）、外切酶exo—β-1，4-glucanase，CBH）、纤维二糖酶（cellobiase，CB）酶活。在5L发酵罐中采用pH控制和流加葡萄糖工艺，可提高CB酶含量，改变酶组分之间的比例，使得FPA、EG、CB和CBH酶活分别达到50．0U／mL，210．0U／mL，4．0U／mL和2．5U／mL，比摇瓶发酵分别提高了6．7．4．2、19、2．5倍。
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| 关 键 词： | 纤维素酶 pH控制 绿色木霉 流加发酵工艺 |
Cellulase production by Trichoderma viride in fed-batch culture under controlled pH condition |
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| GENG Bing,GUO Mei-jin,ZHANG Si-ling,WANG Yong-hong,CHU Ju,ZHUANG Ying-ping.Cellulase production by Trichoderma viride in fed-batch culture under controlled pH condition[J].Industrial Microbiology,2009,39(4):21-26. |
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| Authors: | GENG Bing GUO Mei-jin ZHANG Si-ling WANG Yong-hong CHU Ju ZHUANG Ying-ping |
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| Institution: | (State Key Laboratory of Biore.actor Engineering,East China University of Science ＆ Technology, Shanghai 200237) |
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| Abstract: | Cellulase production by Trichoderma viride was investigated with glucose and (NH_4)_2SO_4 feeding under controlled pH condition. Enzyme activities of filter paper activity(FPA), endo-β-1,4-glucanase(EG), cellobiase(CB) and exo-β-1,4-glucanase(CBH) were 50.0 U/mL,210.0 U/mL,4.0 U/mL and 2.5 U/mL, respectively, in 5 L fermenter culture with pH control and glucose feeding strategy, which were 6.7, 4.2, 19, 2.5 times of that in shaking flask. |
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| Keywords: | cellulose pH control Trichoderma viride fed-batch fermentation |
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