营养条件对光滑球拟酵母积累α-酮戊二酸的影响

以光滑拟球酵母为研究模型，研究α-酮戊二酸的浓度情况。通过单因素实验得到α-酮戊二酸积累最佳浓度的各单因素条件为：葡萄糖浓度140g／L，NH4Cl浓度5g／L。在碳源（30g/L葡萄糖初始浓度）匮乏条件下加入丙酮酸30g／L，在此条件下丙酮酸转化为α－酮戊二酸的转化率最高达53．7％。以30g／L丙酮酸为唯一碳源时在7L发酵罐中光滑拟球酵母可生成浓度为10．7g/Lα-酮戊二酸，外源丙酮酸的转化率可达66．9％。这一结果表明，T．glabrata具有将丙酮酸转化为α-KG的能力。

Effects of nutrient conditions on production of α-ketoglutarate by Torulopsis glabrata

In order to increase the concentration of α-ketoglutarate (α-KG) by Torulopsis glabrata,the optimum concentrations of carbon and nitrogen sources were determined:140 g/L of glucose concentration,5 g/L of NH4Cl concentration.Under the condition when carbon source(30 g/L of the glucose concentration) was short,30 g/L of pyruvate concentration was added and 53.7% of pyruvate could be turned into α-KG.Furthermore,the ability of redistributed carbon source from pyruvate to α-KG by T.glabrata was carefully investigated.It was found that T.glabrata could utilize pyruvate as sole carbon source to synthesize α-KG without any glucose presented in the culture broth.10.7 g/L of α-KG was detected when 30 g/L of pyruvate was fed to the medium and the yield of α-KG from pyruvate was 66.7%.