| 高产β-葡萄糖苷酶N1菌株的分类鉴定 |
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| 引用本文： | 汪大敏,杨国武,李皎,汪允嘉,万一,张琨.高产β-葡萄糖苷酶N1菌株的分类鉴定[J].工业微生物,2012,42(5):57-61. |
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| 作者姓名： | 汪大敏 杨国武 李皎 汪允嘉 万一 张琨 |
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| 作者单位： | 1. 陕西省微生物研究所,西安,710043
2. 陕西省第二人民医院,西安,710005 |
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| 基金项目： | 西安市科技局应用发展研究项目（编号：YF07077）. |
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| 摘 要： | 从纳豆中分离纯化得到1株β-葡萄糖苷酶高产菌株,命名为N1菌,利用16S rDNA分析对该菌作了系统进化鉴定.首先提取N1菌基因组DNA,根据不同种属细菌的16S rDNA序列两端的保守性设计通用引物,对N1菌16S rDNA片断进行PCR扩增,对扩增得到的目标片段测序,利用BLAST对测序结果进行比对,构建系统进化树进行分析,初步确定该菌属于枯草芽孢杆菌.研究表明以16S rDNA分析对该菌鉴定完全可行.
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| 关 键 词： | β-葡萄糖苷酶 16S rDNA 分类鉴定 |
Classification and identification of high producing β-glucosidase strain N~ |
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| WANG Da-min
,
YANG Guo-wu
,
LI Jiao
,
WANG Yun-jia
,
WAN Yi
,
ZHANG Kun.Classification and identification of high producing β-glucosidase strain N~[J].Industrial Microbiology,2012,42(5):57-61. |
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| Authors: | WANG Da-min YANG Guo-wu LI Jiao WANG Yun-jia WAN Yi ZHANG Kun |
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| Institution: | 1. Shaanxi Microbiology Institute, Xi＇ an 710043 ; 2. The Second People＇ s Hospital of Shaanxi Province, Xi＇ an 710043 ) |
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| Abstract: | A strain with high producing β-glucosidase was isolated from natto and was named N1. Identification of the strain was performed using 16S rDNA sequence analysis. First the genomic DNA from N1 was extracted, and then the general primers were designed according to conservative sequence of bacteria 16S rDNA. Furthermore, the target 16S rDNA sequence was amplified and the sequence was analyzed by using the BLAST database. The strain was preliminarily identified as the Bacillus subtilis. The result suggested that the identification of bacteria by 16S rDNA was feasible. |
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| Keywords: | β-glucosidase 15S rDNA identification |
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