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华根霉脂肪酶有机相合成酶活的研究
引用本文:颜兴和,王栋,徐岩.华根霉脂肪酶有机相合成酶活的研究[J].工业微生物,2005,35(2):24-28.
作者姓名:颜兴和  王栋  徐岩
作者单位:江南大学生物工程学院教育部工业生物技术重点实验室,江苏,无锡,214036
摘    要:通过比较7种微生物脂肪酶的有机相合成酶活、水相水解酶活及在正庚烷中催化己酸乙酯合成的能力,证明了合成酶活与水解酶活相关性不高,合成酶活比水解酶活更能反映脂肪酶的合成能力。通过比较两株华根霉(Rhizopus chinensis)脂肪酶酶活,发现合成酶活相差较大,表明相同种属微生物的脂肪酶合成酶活存在不同。对.Rhizopus chinensis-2液态发酵产脂肪酶进程研究发现,水解酶活高峰先于合成酶活高峰大约12h。将不同培养时间的Rhizopus chinensis-2全细胞脂肪酶用于催化己酸乙酯合成,具有高合成酶活的全细胞脂肪酶催化己酸乙酯合成反应较快。因此,全细胞脂肪酶用于催化有机相酯合成反应时,具有高脂肪酶合成酶活的菌体具有较好的催化酯合成能力。

关 键 词:华根霉脂肪酶  有机相合成酶  酶活性  酯合成反应  非水相反应  液态发酵

Synthetic activity of Rhizopus chinensis lipase in organic media
YAN Xing-He,WANG Dong,XU Yan.Synthetic activity of Rhizopus chinensis lipase in organic media[J].Industrial Microbiology,2005,35(2):24-28.
Authors:YAN Xing-He  WANG Dong  XU Yan
Abstract:Comparison of synthetic activities in organic media and hydrolytic activities in aqueous media of lipases from seven microorganisms proved an absence of general correlation between synthetic activity and hydrolytic activity. When lipases from seven microorganisms were used to catalyze synthesis of ethyl hexanoate, synthetic activity was more adapt to indicate lipase esterification ability compared hydrolytic activity. Mensurated the activities of lipases from two Rhizopus chinensis, it was found their synthetic activities were more different and showed synthetic activities of lipases from the same genus microorganisms were different. During lipase liquid fermentation process of Rhizopus chinensis-2, hydrolytic activity production peak was ahead of synthetic activity production peak about 12 hours. Rhizopus chinensis -2 whole-cell lipases of different culture time were used to catalyze synthesis of ethyl hexanoate and whole-cell lipase with high synthetic activity catalyzed esterification quickly. So cell used to catalyze esterification in organic media with high synthetic activity had good esterification ability.
Keywords:lipase  Rhizopus chinensis  synthetic activity  esterification
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