| 不同pH调节剂对补料批式发酵产1,3-丙二醇的影响 |
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| 引用本文： | 唐浩,郭美锦,庄英萍.不同pH调节剂对补料批式发酵产1,3-丙二醇的影响[J].工业微生物,2012,42(3):45-49. |
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| 作者姓名： | 唐浩 郭美锦 庄英萍 |
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| 作者单位： | 唐浩 (华东理工大学国家生化工程技术研究中心,上海,200237) ； 郭美锦 (华东理工大学国家生化工程技术研究中心,上海,200237) ； 庄英萍 (华东理工大学国家生化工程技术研究中心,上海,200237) ； |
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| 摘 要： | 对肺炎克雷伯氏菌（Klebsiellapneumoniae）发酵生产1，3-丙二醇（1，3-Propanediol，1，3．PD）的补碱策略进行了研究。分别利用NaOH、氨水、KOH三种溶液作为pH调节剂，优化三种pH调节剂并得到按一定比例混合的混合碱。当采用混合碱调控发酵pH值为7．0时，1，3-丙二醇的产量达到了55dL，比无pH调控（对照）发酵过程发酵水平提高了10．6倍。
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| 关 键 词： | 肺炎克雷伯氏菌 1，3-丙二醇 pH调节剂 补料批式发酵 |
Effects of different pH regulators on 1,3-propanediol production by Klebsiella pneumoniae in fed-batch culture |
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| TANG Hao,GUO Mei-iin,ZHUANG Ying-ping.Effects of different pH regulators on 1,3-propanediol production by Klebsiella pneumoniae in fed-batch culture[J].Industrial Microbiology,2012,42(3):45-49. |
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| Authors: | TANG Hao GUO Mei-iin ZHUANG Ying-ping |
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| Institution: | (National Engineering Center for Biotechnology, East China University of Science and Technology, Shanghai 200237) |
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| Abstract: | Bioprocess optimization and pH control strategies for 1,3-propanediol （1,3-PD） production by Klebsiella pneumoniae were investigated, 3 kinds of pH regulator i. e. NaOH, ammonia and KOH solutions were optimized respectively and then a mixed alkaline solution in certain proportion was used for maintaining pH 7.0 in fed-hatch fermentation. The maximum 1,3-PD level of 55 g/L was obtained with the mixed alkaline solution, which was 10.6 times than that of the control without pH adjustment. |
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| Keywords: | Klebsiella pneumon/ae 1 3-propanediol pH regulator fed-batch fermentation |
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