| 纺锤芽孢杆菌CGMCC1347发酵生产异丁香酚单加氧酶的条件优化 |
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| 引用本文： | 赵丽青,肖向东,孙志浩,方佳茂,陈伟滨.纺锤芽孢杆菌CGMCC1347发酵生产异丁香酚单加氧酶的条件优化[J].工业微生物,2012,42(4):60-64. |
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| 作者姓名： | 赵丽青 肖向东 孙志浩 方佳茂 陈伟滨 |
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| 作者单位： | 赵丽青 (深圳大学化学与化工学院食品科学系) ； 肖向东 (深圳大学医学院) ； 孙志浩 (江南大学生物工程学院) ； 方佳茂 (广东环西生物科技股份有限公司) ； 陈伟滨 (广东环西生物科技股份有限公司) ； |
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| 基金项目： | 深圳大学基础研究科研项目 |
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| 摘 要： | 对从土壤中筛选获得的纺锤芽孢杆菌CGMCCl347生产异丁香酚单加氧酶的发酵条件进行了单因素考察及正交实验优化，确定了最适的发酵摇瓶培养基组成和培养条件。在发酵培养基组成为尿素1g／L，玉米浆55g/L，K2HP042g／L，MgSO4·7H2O1g／L，初始pH7．5，发酵温度37℃，摇床转速180r／min的条件下培养16h获得的细胞，能转化2％的异丁香酚生成2．49g／L香兰素，异丁香酚单加氧酶酶活达3．79U／L。
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| 关 键 词： | 异丁香酚单加氧酶 香草醛 纺锤芽孢杆菌 发酵 |
Optimization of fermentation conditions for isoeugenol monooxygenase from Bacillus fusiformis CGMCC1347 |
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| ZHAO Li-qing,XIAO Xiang-dong,SUN Zhi-hao,FANG Jia-mao,CHEN Wei-bin.Optimization of fermentation conditions for isoeugenol monooxygenase from Bacillus fusiformis CGMCC1347[J].Industrial Microbiology,2012,42(4):60-64. |
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| Authors: | ZHAO Li-qing XIAO Xiang-dong SUN Zhi-hao FANG Jia-mao CHEN Wei-bin |
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| Institution: | 1. Department of Food Science and Engineering, College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, Guangdong 518060 ; 2. School of Medicine, Shenzhen University, Shenzhen, Guangdong 518060 ; 3. School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122; 4. Guangdong Huanxi Biological Technology CO., LTD, Puning, Guangdong 515343) |
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| Abstract: | optimized Fementaition conditions for Bacillus fusiformis CGMCC1347 producing isoeugenol monooxygenase were through single and orthogonal experiments. The suitable fermentation conditions were determined as follows： 2.49 g/L vanillin was obtained from 20 g/L isoeugenol with the cells cultured at initial pH 7.5,37℃, 180 r/min for 16 h in the fermentation culture containing urea 1 g/L, K2HPO4 2 g/L, corn steep liquid 55 g/L, and MgSO4 ·7H20 1 g/ L. Under the optimized conditions, the isoeugnol monooxygenase activity reached 3.79 U/L. |
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| Keywords: | isoeugenol monooxygenase vanillin Bacillus fusiformis fermentation |
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