利用黑曲霉固态发酵啤酒糟生产饲料复合酶的研究

以啤酒糟为主要基质,利用黑曲霉固态发酵生产酸性蛋白酶、木聚糖酶和纤维素酶等多种饲料复合酶,研究了黑曲霉固态发酵培养基组成对复合酶酶活的影响,确定最优培养基配方为:啤酒糟75%,麸皮25%,硫酸铵1%,KH_2PO_4 0.2%,MnSO_4 0.1%、ZnSO_4 0.2%,料水比1:2。在适宜的发酵条件下,经30℃发酵5 d,烘干后得到的复合酶制剂中,具有多种酶活性(以干基计)。其中酸性蛋白酶活力3 800 U/g,木聚糖酶活力12 00 U/g和纤维素酶活力18 U/g。

Production of feed compound enzymes from brewers grain through solid fermentation by Aspergillus niger

The solid state fermentation for production of feed compound enzymes from beer spent grains by Aspergillus niger and the effects of medium compositions on feed compound enzymes were investigated. As a result, the optimal medium compositions were as follows： beer spent grains 75%, wheat bran 25%, （NH4）2SO4 1%, KH2PO4 0.2%, MnSO4 0.05 %, Zn,KH2PO4 0.2 %, ratio of raw material to water 1 ： 2. After cultivation the activities of compound enzymes were measured as acid protease activity 3 800 U/g, xylanase activity 1 200 U/g and cellulose enzyme activity 18 U/g.