根霉纤溶酶发酵条件的优化

研究了不同培养基对中国根霉１２号（Ｒｈｉｚｏｐｕｓ ｃｈｉｎｅｓｉｓ１２）发酵生产纤溶酶的影响，发现Ｃ／Ｎ的降低有利于产酶，同时用正产实验给出了最佳培养条件为：以麸皮水＋胰蛋白胨＋豆渣为培养基，接种量２０％，转速１７０ｒ／ｍｉｎ；用响应面设计确定了最佳培养基为４．３６６６１％麸皮水＋０．９８９７５％胰蛋白胨＋４．８９８９５％豆渣；在最优条件下，纤溶酶的发酵效价可达３．０６Ｕ／ｍｌ。发现添加金属离子

Optimization of fermentation conditions of fibrinolytic enzyme produced by Rhizopus chinesis sp.

The fermentation conditions of fibrinolytic enzyme produced by Rhizopus chinesis 12 were discussed . Compared with different media it was found that the decrease of C/N would facilitate the generation of fibrinolytic enzyme. The optimum fermentation conditions determined by orthogonal test and response surface experiment (Box-Behnken Designs) were as follows: inoculum size 20%, rotation speed 170 r/min and the optimum medium consisted of 4.36661 % of bran, 0.98975 % of tryptone and 4.89895 % of soybean residue. Under the optimum cultivation conditions, the fermentation titer could approach 3.06 U/ml. The output was improved by adding metal ions such as Ca 2 , Mg 2 . And, the changes of different parameters during fermentation were shown by curves.