| 培养条件对赭曲霉菌球形成和坎利酮11α羟基化的影响 |
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| 引用本文： | 谢进霞,别松涛,刘逸寒,徐星,李建勋,刘晓,路福平.培养条件对赭曲霉菌球形成和坎利酮11α羟基化的影响[J].工业微生物,2011,41(6):6-10. |
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| 作者姓名： | 谢进霞 别松涛 刘逸寒 徐星 李建勋 刘晓 路福平 |
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| 作者单位： | 工业发酵微生物教育部重点实验室,工业酶国家工程实验室,天津市工业微生物重点实验室,天津科技大学生物工程学院,天津,300457 |
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| 摘 要： | 菌丝粗壮、分布均匀的菌球可提高赭曲霉11α羟基化的效率.控制赭曲霉菌球直径的平均大小可以通过选择不同的培养温度、装液量、以及转速等因素而达到.通过正交分析,确定培养温度为30 ℃,按孢子悬液浓度为108个/mL接种,装液量为50 mL/250 mL三角瓶,摇床转速为200 r/min时,菌球达到最佳直径2～3 mm之间...
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| 关 键 词： | 赭曲霉 菌球形态 坎利酮11α羟基化 转化率 |
Effects of culture conditions on pelletes growth form of Aspergillus ochraceus for canrenone 11α hydroxylation |
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| XIE Jin-xia,BIE Song-tao,LIU Yi-han,XU Xing,LI Jian-xun,LIU Xiao,LU Fu-ping.Effects of culture conditions on pelletes growth form of Aspergillus ochraceus for canrenone 11α hydroxylation[J].Industrial Microbiology,2011,41(6):6-10. |
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| Authors: | XIE Jin-xia BIE Song-tao LIU Yi-han XU Xing LI Jian-xun LIU Xiao LU Fu-ping |
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| Institution: | (Key Laboratory of Industrial Microbiology, Ministry of Education, National Engineering Laboratory for Industrial Enzymes, Tianjin Key Laboratory of Industrial Microbiology,College of Biotechnology, Tian jin University of Science＆ Technology, Tian jin 300457, China) |
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| Abstract: | The efficiency of 11α hydroxylation could be improved by Aspergillus ochraceu pellets with thick hyphae and uniform distribution. The experiment indicated that the mean size of pellets could be controlled by different culture temperature, inoculation volume, rotation speed and other factors. Through the orthogonal analysis, when the culture temperature was 30 ℃, the concentration of spore suspension was inoculated with 108 spores/mL, medium volume 50 mL/250 mL flask, shaker speed 200 r/min, the maximum rate of the pellet diameter from 2 mm to 3 mm was obtained. Under these conditions by adding 10 g/L of canrenone, the final conversion rate reached as high as 91.26 %. |
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| Keywords: | Aspergillus ochraceus pelletes morphology canrenone 11α-hydroxylation bioconversion rate |
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