布雷菲尔德菌素A高产菌的选育和发酵条件优化

研究了选育布雷菲尔德茵素A(BFA)高产茵和优化发酵条件.采用紫外线照射(UV)、亚硝基胍(NTG)和UV+NTG复合诱变处理BFA产生茵Eupenicillum sp.E-0506筛选突变株后进一步发酵选育;采用单因素筛选结合正交试验考察摇瓶装液量、转速等因素的影响.试验所确定的BFA产生茵的孢子诱变适宜条件为:孢子...

Breeding of a high brefeldin A -producing strain and its optimization of fermentation conditions

The breeding of high brefeldin A-producing strains and optimization of fermentation conditions were investigated. Eupenicillum sp. E-0506 used as an original strain for mutagenetic breeding by LIV, NTG and UV ＋ NTG, respectively. The fermentation conditions were optimized by single factor test and orthogonal test. The spore sus- pension of E-0506 was treated by UV irradiation for 60 seconds, followed with 2.0 mg/mL NTG for 30 minutes under magnetic stirring. A mutant Eupenicillum sp. E-UN88 producing brefeldin A （BFA） with high yield and well hereditary stability was obtained. Its optimum fermentation parameters were as follows： medium volume 100 mL/500 mL, inoculum size 5 %, cultivation time of seed 43 h and fermented at 26 ~2, 250 r/min, for 120 h. The BFA yield of Eupenicillum sp. E-UN88 was enhanced more than 10.36 times higher than that of the original strain. The results laid a foundation of the brefeldin A production of industrial fermentation.