| L-天冬氨酸-β-脱羧酶发酵条件的响应面优化 |
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| 引用本文： | 薛正莲,高理所,赵世光,赵梦梦,苏燕南.L-天冬氨酸-β-脱羧酶发酵条件的响应面优化[J].工业微生物,2011,41(6):11-14. |
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| 作者姓名： | 薛正莲 高理所 赵世光 赵梦梦 苏燕南 |
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| 作者单位： | 安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽,芜湖,241000 |
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| 摘 要： | 利用Plackett-Burman设计和响应面分析方法对L-天冬氨酸-β-脱羧酶产生菌德阿昆哈假单孢菌XG-2-81的发酵条件进行优化.得出影响产L-天冬氨酸-β-脱羧酶的3个重要的因素为:玉米浆,多肽朊和装液量.通过对实验结果的方差分析,得到其最适条件分别为:玉米浆浓度0.64%,多肽朊浓度1.21%,装液量14.7...
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| 关 键 词： | 响应面设计法 L-天冬氨酸-β-脱羧酶 德阿昆哈假单孢菌 优化 |
Optimization of fermentation conditions for L-aspartic acid -β-decarboxylase by response surface methodology |
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| XUE Zheng-lian,GAO Li-suo,ZHAO Shi-guang,ZHAO Meng-meng,SU Yan-nan.Optimization of fermentation conditions for L-aspartic acid -β-decarboxylase by response surface methodology[J].Industrial Microbiology,2011,41(6):11-14. |
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| Authors: | XUE Zheng-lian GAO Li-suo ZHAO Shi-guang ZHAO Meng-meng SU Yan-nan |
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| Institution: | (Institute of Biologic ＆ Chemical Engineering of Anhui Polytechnic University, Microorganism fermentation engineering and technology research center of anhui province, Wuhu , Anhui 241000, China) |
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| Abstract: | The Plackett-Burman design and response surface analysis methods were used to optimize the fermentation conditions of L-aspartic acid-β-decarboxylase producing by Pseudomonas dacunhae XG-2-81. The corn steep liquor, prion peptides and medium volume were found to be the important factors. Based on the experimental results of analysis of variance, the optimum conditions were as follows： 0.64% corn steep liquor, 1.21% prion peptides, 14.71% liquid vol- ume. The activity of L-aspartic acid-β-deearboxylase could reach 1107 μg / mL h by using the optimal fermentation conditions, which was increased by 10.83 % compared with that by the original conditions. |
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| Keywords: | response surface methodology L-aspartic acid-β-decarboxylase Pseudomonas dacunha optimization |
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