| 产β-葡萄糖苷酶菌株的筛选鉴定及其离子束诱变 |
| |
| 引用本文： | 郭慧女,徐岩,喻晓蔚,康文怀.产β-葡萄糖苷酶菌株的筛选鉴定及其离子束诱变[J].工业微生物,2011,41(5):32-37. |
| |
| 作者姓名： | 郭慧女 徐岩 喻晓蔚 康文怀 |
| |
| 作者单位： | 江南大学生物工程学院酿造科学与酶技术中心教育部工业生物技术重点实验室,江苏无锡,214122 |
| |
| 基金项目： | 教育部长江学者和创新团队发展计划 |
| |
| 摘 要： | 利用经过改良的初筛培养基,以p-NPG为底物作为筛子,从葡萄园土壤及葡萄表皮中筛选到一株产β-葡萄糖苷酶酶活较高的茵株,经18S rDNA鉴定为黑曲霉.通过离子束注入技术对该茵株进行诱变,获得了一株高产β-葡萄糖苷酶诱变菌株H68,与原茵株相比,酶活提高了53%.
|
| 关 键 词： | β-葡萄糖苷酶 黑曲霉 N+离子束 |
Screening and identification of a high β-glucosidase producing strain and its ion beams mutation |
| |
| GUO Hui-nv,XU Yan,YU Xiao-wei,KANG Wen-huai.Screening and identification of a high β-glucosidase producing strain and its ion beams mutation[J].Industrial Microbiology,2011,41(5):32-37. |
| |
| Authors: | GUO Hui-nv XU Yan YU Xiao-wei KANG Wen-huai |
| |
| Institution: | (Key Laboratory of Industrial Biotechnology, Ministry of Education, Center of Brewing Science and Enzyme Technology, school of Biotechnology, jiangnan university,wuxi 214122, China) |
| |
| Abstract: | A novel srtain for producing β-glucosidases was isolated from soil and gapes by modified medium and was identified as Aspergillus niger by 18S rNDA. The strain was mutated by low-energy ion implantation and a genetically stable mutant H68 was obtained. Compared with the wild strain, its enzyme activity was improved 53 %. |
| |
| Keywords: | β-glucosidases Aspergillus niger N＋ ion beam |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
