菊糖芽孢乳杆菌DS2-18中试规模的D-乳酸发酵研究

研究了菊糖芽孢乳杆茵DS2的突变株DS2-18在中试规模的D-乳酸发酵.在容积为300L自控发酵罐中,DS2-18茵在合适的发酵条件下,即培养基组成(g/L):葡萄糖120,玉米浆8,蛋白胨6,豆粕水解液100,接种量8%(v/v),发酵温度40℃,以轻质碳酸钙作为中和剂调pH 5～6,发酵期间交替不通气和通气,发酵6...

D-lactic acid production on pilot scale by Sporolactobacillus inulinus DS2-18

D-lactic acid production on pilot scale by DS2-18, a mutant of Sporolactobacillus inulinus DS2, was investigated. Its fermentation was carried out in a 300 L automatic control fermentor. The medium were as follows （g/L）： glucose 120, corn steep liquor 8, peptone 6, soybean meal hydrolysate （SMH） 100. Under the proper conditions such as inoculum size 8% （v/v）, fermentation temperature 40 ℃, pH 5--6 with light calcium carbonate as neutralizer, alternative aeration or no aeration in the course of fermentation duration, the concentration of D-lactic acid , the yield and productivity were 109.4 %, 93.5 % and 1.60 g/L· h, respectively, and the optical purity of D-lactic acid was 99.0 % . Meanwhile, SMH, instead of expensive yeast extracts, could reduce the manufacturing cost for producing D-lactic acid. As a result, it laid a foundation of mass production of D-lactic acid.