| N~+离子注入选育高产氨基酰化酶菌株的研究 |
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| 引用本文： | 刘丽华,刘丽萍,李文彬,王霞.N~+离子注入选育高产氨基酰化酶菌株的研究[J].工业微生物,2010,40(2):58-61. |
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| 作者姓名： | 刘丽华 刘丽萍 李文彬 王霞 |
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| 作者单位： | 内蒙古工业大学,化工学院,内蒙古,呼和浩特市,010051 |
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| 基金项目： | 内蒙古自然科学基金资助项目 |
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| 摘 要： | 选用N~+离子注入的方法对米曲霉(Aspergillus oryzae)CICC 2339-1进行诱变育种,通过三角瓶发酵法筛选氨基酰化酶高产株。N~+离子注入选择能量为10 KeV,剂量在(1.30～4.94)×10~(15)ions/cm~2之间。根据剂量与存活率以及剂量与突变率曲线选择最佳的注入剂量。通过三角瓶发酵筛选得到突变菌株SN-110-15其酶活提高率为139.5%,诱变试验效果显著。
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| 关 键 词： | 米曲霉 离子注入 氨基酰化酶 |
Breeding of aminoacylase producing strain by N+ ion implantation |
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| LIU Li-hua,LIU Li-ping,LI Wen-bin,WANG Xia.Breeding of aminoacylase producing strain by N+ ion implantation[J].Industrial Microbiology,2010,40(2):58-61. |
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| Authors: | LIU Li-hua LIU Li-ping LI Wen-bin WANG Xia |
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| Institution: | (School of Chemistry Engineering and Technology, Inner Mongolia University of Technology, Huhhot 010051, Inner Mongolia, China) |
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| Abstract: | In order to obtain the aminoaeylase high-producing strain, Aspergillus oryzae CICC 2339-1 was mutagenized by N^＋ ion implantation method. Ion energy implanted was 10 KeV and its dose was ranged from 1.30×10^15 ions/cm^2 to 4.94 10^15 ions/cm^2. In accordance with the survival rate curve and the mutation rate curve of Aspergillus oryzae caused by ion beam implantation, the optimum implanting dose was found. The aminoacylase high-producing strain SN-110-15 was obtained by fermentation screening method and the enzyme activity was enhanced 139.5 % than that of the parent strain. The effect of mutation was obvious. |
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| Keywords: | Aspergillus oryzae ion implantation aminoacylase |
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