| 产共轭亚油酸菌株的筛选及其等离子体诱变 |
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| 引用本文： | 孙丽慧,王庆山,赵淑艳,修志龙.产共轭亚油酸菌株的筛选及其等离子体诱变[J].工业微生物,2016(5):50-54. |
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| 作者姓名： | 孙丽慧 王庆山 赵淑艳 修志龙 |
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| 作者单位： | 1. 大连理工大学食品与环境学院,辽宁盘锦,124221;2. 大连理工大学生命科学与技术学院,辽宁大连,116024 |
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| 基金项目： | 大连理工大学基本科研业务费专项项目(DUT16QY31)。 |
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| 摘 要： | 本实验从东北酸菜汁中筛选获得了一株产共轭亚油酸能力较好的菌株SC-05,特别是气相色谱分析结果表明,合成的CLA产物构型为单一构型,仅为c9, t11-18：2-CLA,经鉴定该菌为乳杆菌属Lactobacillus sp.。菌株SC-05经低温等离子体处理后,筛选出突变株A-08,其共轭亚油酸产量达到119.24μg/mL,比出发菌株增加了83.0%,且突变株A-08具有较好的遗传稳定性。
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| 关 键 词： | 共轭亚油酸 筛选 乳杆菌属 等离子体诱变 |
Screening and plasma mutagenesis of conjugated linoleic acid high-producing strain |
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| Abstract: | The strain SC-05 with the ability of synthesize conjugated linoleic acid ( CLA ) was isolated from sauerkraut juice samples. According to gas chromatography analysis, c9, t11-CLA was the only product in the fermentation broth. This strain was identified as Lactobacillus sp. , based on full-length 16S rDNA. The strain SC-05 was induced by low temperature plasma, and the mutant strain A-08 was obtained. The results revealed that the CLA production by the mutant strain A-08 could reach 119. 24 μg/mL, raised 83. 0% compared with that by the original strain. And the mutant strain A-08 also exhibited its good genetic stability. |
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| Keywords: | conjugated linoleic acid screening Lactobacillus plasma mutagenesis |
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