| L-鸟氨酸的混合菌种发酵研究 |
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| 引用本文： | 李环,周磊,陈悦,韦萍,欧阳平凯.L-鸟氨酸的混合菌种发酵研究[J].工业微生物,2006,36(4):37-42. |
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| 作者姓名： | 李环 周磊 陈悦 韦萍 欧阳平凯 |
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| 作者单位： | 南京工业大学制药与生命科学学院,南京,210009 |
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| 基金项目： | 致谢：感谢本学院分析实验室的于文涛老师在GC/MS和博士生李晖在HPLC方面给予的大力支持及帮助! |
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| 摘 要： | 谷氨酸棒杆菌可发酵葡萄糖产L-鸟氨酸。通过连续筛选与驯化筛选到了产量较高的生产菌株CG1006。以GC/MS对发酵液成分的定性分析及鸟氨酸的产量为依据,优化了培养条件,产量从最初的14.346g/L提高到40.416g/L。初步考察了酵母菌CG1006-1对发酵液成分及产量的影响。结果表明混合菌种发酵可纯化发酵液成分。
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| 关 键 词： | L-鸟氨酸 发酵 谷氨酸棒杆菌CG1006 酵母菌CG1006-1 纯化 |
Studies on multi-bacterium fermentation of L-ornithine |
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| LI Huan,ZHOU Lei,CHEN Yue,WEI Ping,OUYANG Pin-kai.Studies on multi-bacterium fermentation of L-ornithine[J].Industrial Microbiology,2006,36(4):37-42. |
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| Authors: | LI Huan ZHOU Lei CHEN Yue WEI Ping OUYANG Pin-kai |
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| Institution: | College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009 |
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| Abstract: | L-ornithine can be produced from glucose by Corynebacterium Glutamicum. A high yield strain CG1006 was obtained by many times of selection and cultivation.On the bases of the qualitative analysis of fermentation broth by GC-MS and the quantitative analysis by HPLC,the culture medium was optimized.And the yield was improved from(14.346)g/L to 40.416 g/L.The effects of yeast 1006-1 on the L-ornithine yield and the fermentation component were(investigated.) A new idea of coupling technology between fermentation and purification was put forward. |
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| Keywords: | L-orinine fermentation CG 1006 Yeast 1006-1 purification |
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