植物乳杆菌粘附性与其表面特征关系的探究

本文通过16s rDNA鉴定获得4株植物乳杆菌,并以HT29细胞为体外黏附筛选模型,进一步探讨了这些菌株粘附能力与表面疏水性、自聚共聚能力等表型特征的相关性。结果表明,植物乳杆菌AR326菌株对HT29细胞的粘附性最强,并显示高度的自聚性(25%)和共聚性(25%),但其表面疏水性偏低(15%);通过相关性分析发现,植物乳杆菌的自聚性和共聚性与HT29细胞粘附性呈显著相关性(r=1.0和0.8,p0.05),但表面疏水性、自凝聚性和共聚性两两之间并无显著相关性(p0.05)。本研究结果为建立快速筛选高粘附性植物乳杆菌的方法及其菌株在体内定植和分布研究提供一定参考依据。

Relationships between adhesion abilities and surface properties of Lactobacillus plantarum

Four Lactobacillus plantarum strains were isolated and identified from fermented food using HT29 cells as in vitro model combined with 16S rDNA sequencing identification.The correlation of phenotypic characteristics of Lactobacillus plantarum strain adhesion ability,surface hydrophobicity and aggregation ability were further investigated.Results indicated that AR326 of Lactobacillus plantarum showed the strongest adhesion to the HT29 cells.Furthermore,this strain also exhibited highly aggregation ability (the aggregation rate at 5 h ＞ 25％) and highly co-aggregation ability (the co-aggregation rate at 5 h ＞25％).However,its surface hydrophobic ability (the surface hydrophobicity ＜ 15％) was unexpectedly lowest among the four strains.Correlation analysis results revealed that the auto-aggregation ability and co-aggregation ability of Lactobacillus plantarum from fermented food were related to the adhesive ability of the HT29 cells significantly (p ＜0.05);but there were not significantly (p ＞ 0.05) correlated to each other between auto-aggregation ability,co-aggregation ability and surface hydrophobicity.These results provided a reference for establishing techniques of rapid screening highly adhesive Lactobacillus plantarum strains and their colonization and distribution in vivo.