| 培养条件对里氏木霉306菌体形态和t-PA生物合成的影响 |
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| 引用本文： | 江洁,杜连祥,路福平,姚蕾,于海龙,江成英,曹畅.培养条件对里氏木霉306菌体形态和t-PA生物合成的影响[J].工业微生物,2005,35(4):9-14. |
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| 作者姓名： | 江洁 杜连祥 路福平 姚蕾 于海龙 江成英 曹畅 |
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| 作者单位： | 天津科技大学食品科学与生物工程学院,天津,300222;齐齐哈尔大学生命科学与工程学院,齐齐哈尔,161006;天津科技大学食品科学与生物工程学院,天津,300222;齐齐哈尔大学生命科学与工程学院,齐齐哈尔,161006 |
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| 基金项目： | 黑龙江省自然科学基金资助项目（No.130352）. |
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| 摘 要： | 里氏木霉（Trichoderrna reesei）306是能够生物合成组织型纤溶酶原激活剂（t-PA）的基因工程菌株。在对其液态深层培养时，发现随培养夸件和培养时间的变化，其菌体能以松散和菌丝球的两种形态存在。菌体形态和t-PA的产生密切相关。培养基中无机盐和表面活性剂的种类和添加量以及接种量和pH等培养条件是影响里氏木霉306菌体形态和t-PA合成的主要因素。在液态深层培养过程中，菌体以松散的菌丝体形态生长，形成纸浆状发酵液，利于t-PA的合成。
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| 关 键 词： | 丝状真菌 里氏木霉 组织型纤溶酶原激活剂 菌体形态 |
Influence of culture conditions on mycelium morphology and t-PA production by Trichoderma reesei 306 |
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| GANG Jie,DU Lian-xiang,LU Fu-ping,YAO Lei,YU Hai-long,JIANG Cheng-ying,CAO Chang.Influence of culture conditions on mycelium morphology and t-PA production by Trichoderma reesei 306[J].Industrial Microbiology,2005,35(4):9-14. |
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| Authors: | GANG Jie DU Lian-xiang LU Fu-ping YAO Lei YU Hai-long JIANG Cheng-ying CAO Chang |
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| Institution: | 1. The College of Food Science and Biotechnology , Tianjing Science and Technology University, Tianjing 300222; 2. The College of Life Science and Technology , Qiqihar University, Qiqihar 161006 |
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| Abstract: | Trichoderma reesei 306 was a recombinant strain that can produce tissue-type plasminogen activator(t-PA).During submerged cultivation,the morphology of the filamentous fungal was usually two types: pellet and dispersed form.The morphology had relationship with t-PA production.The kind and concentration of metal ions and surfactants in(fermentation) medium,the inoculum size and pH were the main factors influencing the t-PA biosynthesis.In submerged culture course,the dispersed mycelium in growth phase forming pulpy fermentation broth was advantageous to t-PA(production). |
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| Keywords: | filamentous fungal Trichoderma reesei tissue-type plasminogen activator mycelium morphology |
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