| 肺炎克雷伯氏菌荚膜多糖表达量评价指标的建立及发酵条件优化 |
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| 引用本文： | 张燕,李轻舟,杜连祥,戚薇,陈锦英.肺炎克雷伯氏菌荚膜多糖表达量评价指标的建立及发酵条件优化[J].工业微生物,2006,36(2):16-21. |
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| 作者姓名： | 张燕 李轻舟 杜连祥 戚薇 陈锦英 |
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| 作者单位： | 1. 天津科技大学生物工程学院,天津,300222
2. 天津医科大学基础医学院,天津,300070 |
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| 摘 要： | 建立了特异性强的肺炎克雷伯氏菌荚膜多糖全菌ELISA检测方法,检测结果与多糖表达量相关性好;以全菌ELISA值结合菌数为评价指标,对影响荚膜多糖表达的培养基组成及发酵条件进行了优化,优化后的摇瓶培养条件下发酵液活性和生物量分别比优化前提高72.7和33倍,并经7L罐放大实验,绘制发酵动力学曲线,为肺炎克雷伯氏菌荚膜多糖进一步开发打下基础。
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| 关 键 词： | Klebsiella pneumoniae 荚膜多糖 全菌ELISA 发酵条件 |
Foundation of evaluating method of Klebsiella pneumoniae capsular polysaccharide expression and optimization of fermentation process |
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| ZHANG Yan,LI Qing-zhou,DU Lian-xiang,QI Wei,CHEN Jin-ying.Foundation of evaluating method of Klebsiella pneumoniae capsular polysaccharide expression and optimization of fermentation process[J].Industrial Microbiology,2006,36(2):16-21. |
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| Authors: | ZHANG Yan LI Qing-zhou DU Lian-xiang QI Wei CHEN Jin-ying |
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| Institution: | 1. College of Bioengineering, Tianjin University of Science and Technology,TianJin 300222; 2. College of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070 |
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| Abstract: | In order to evaluate the fermentation process of Klebsiella pneumoniae,indirect cell ELISA was established and investigated.It showed a good correlation to the yield of active capsular polysaccharide.Both indirect cell ELISA and biomass were utilized to evaluate the fermentation process.The influence of substrate composition and growth conditions on CPS expression by Klebsiella pneumoniae using submerged cultivation were investigated systematically.The statistical optimization of media and growth conditions in shake flask were established.The biomass and activity of the culture under the optimum process were enhanced by 72.7 and 33 times respectively.Then the process was magnified and improved in stired tank fermenter. |
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| Keywords: | Klebsiella pneumoniae capsular polysaccharide indirect cell ELISA fermentation optimization |
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