| 一株东祁连山耐低温纤维素降解菌株的分离、鉴定及产酶特性 |
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| 引用本文： | 孔雅丽,贾辉,王生荣,陈秀蓉,杨成德,卢光新.一株东祁连山耐低温纤维素降解菌株的分离、鉴定及产酶特性[J].工业微生物,2014,44(1):60-66. |
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| 作者姓名： | 孔雅丽 贾辉 王生荣 陈秀蓉 杨成德 卢光新 |
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| 作者单位： | 孔雅丽 (甘肃农业大学草业学院,草业生态系统重点实验室,甘肃,兰州,730070)； 贾辉 (甘肃农业大学草业学院,草业生态系统重点实验室,甘肃,兰州,730070)； 王生荣 (甘肃农业大学草业学院,草业生态系统重点实验室,甘肃,兰州,730070)； 陈秀蓉 (甘肃农业大学草业学院,草业生态系统重点实验室,甘肃,兰州,730070)； 杨成德 (甘肃农业大学草业学院,草业生态系统重点实验室,甘肃,兰州,730070)； 卢光新 (青海大学农牧学院,草业科学系,青海,西宁,810016)； |
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| 基金项目： | 国家自然科学基金项目(项目编号：41261064) |
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| 摘 要： | 从东祁连山高寒草甸土壤中分离得到一株纤维素酶高产菌株【3-2。根据菌体形态观察、革兰氏染色反应及16S rDNA序列测定以及序列同源性比较,确定鉴定该菌为芽孢杆菌属的成员(Bacillus sp.)。对该菌的产纤维素酶条件研究结果表明:该菌在5℃~45℃、初始pH 4.0~9.0的环境下均能产纤维素酶。在初始pH为8.0、盐浓度为2.0%~3.0%之间、培养温度为20℃培养条件下最适产酶。在5℃时,相对酶活仍能保持60%。
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| 关 键 词： | 高寒草甸 耐低温纤维素酶 芽孢杆菌 产酶特性 |
Isolation,identification and characterization of a cold-adapted cellulose- degrading strain from eastern Qilian mountains |
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| KONG Ya-li,JIA Hui,WANG Sheng-rong.Isolation,identification and characterization of a cold-adapted cellulose- degrading strain from eastern Qilian mountains[J].Industrial Microbiology,2014,44(1):60-66. |
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| Authors: | KONG Ya-li JIA Hui WANG Sheng-rong |
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| Institution: | 2 of Pratacuhure, Gansu Agricultural University, Lanzhou 730070; Animal Husbandry College of Qinghai University, Xining 810016 |
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| Abstract: | The strain X3-2 producing extracellular cellulase was isolated from eastern Qilian mountains and was identified as Bacillus sp. based on its morphological, biochemical physiological properties and 16S rDNA sequence analysis. The results of cellulase production research indicated that the strain X3-2 could produce cellulase at 5 ℃ ~ 45 ℃ 9.0. The most suitable culture conditions for enzyme production were temperature 20 ℃, pH 8.0, NaC1 2.0% Moreover, the relative enzyme activity still kept 60% at 5 ℃. |
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| Keywords: | alpine meadow cold-adapted cellulase Bacillus sp cellulase-producing characteristics pH4 0 -3 0% |
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