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| 一株产几丁质酶菌株的筛选及其产酶条件的研究 | |
| 引用本文： | 李静,刘建军,赵祥颖.一株产几丁质酶菌株的筛选及其产酶条件的研究[J].工业微生物,2007,37(3):44-47. |
| 作者姓名： | 李静 刘建军 赵祥颖 |
| 作者单位： | 1. 青岛农业大学食品科学与工程学院,青岛,266109 2. 山东省食品发酵工程重点实验室,济南,250013;山东轻工业学院食品与生物工程学院,济南,250100 3. 山东省食品发酵工程重点实验室,济南,250013 |
| 摘 要： | 采用平板透明圈法从土壤中分离筛选到一株产几丁质酶放线菌株L12,用250mL摇瓶发酵初筛和复筛,酶活力为0.63U/mL。通过产酶条件实验,初步确定了该菌株较适产酶培养基和摇瓶发酵条件。条件优化后,30℃、250mL摇瓶发酵48h,几丁质酶活力达到1.06U/mL。 |
| 关 键 词： | 几丁质 几丁质酶 筛选 酶活力 |
Screening of chitinase froming strains and conditions for enzyme production |
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| LI Jing,LIU Jian-jun,ZHAO Xiang-ying.Screening of chitinase froming strains and conditions for enzyme production[J].Industrial Microbiology,2007,37(3):44-47. | |
| Authors: | LI Jing LIU Jian-jun ZHAO Xiang-ying |
| Abstract: | L12 strain with high chitinase activity were isolated from soil.The activity in 250mL flask was 0.63U/mL.The optimal culture medium and fermentation conditions were investigated.Under the optimal conditions,strain L12 produced enzyme as high as 1.06 U/mL. |
| Keywords: | chitin chitinase screening enzyme activity |
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