首页 | 本学科首页   官方微博 | 高级检索  
   检索      

酰胺水解酶基因工程菌的构建及其对棘白菌素B的转化
引用本文:常青,邵雷,李继安,倪兵,陈代杰.酰胺水解酶基因工程菌的构建及其对棘白菌素B的转化[J].工业微生物,2011,41(2):11-15.
作者姓名:常青  邵雷  李继安  倪兵  陈代杰
作者单位:1. 华东师范大学生命科学学院,上海200062;上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200040
2. 上海医药工业研究院创新药物与制药工艺国家重点实验室,上海,200040
3. 华东师范大学生命科学学院,上海,200062
摘    要:棘白菌素B0(ECB)去侧链母核为重要抗真菌药物阿尼芬净的半合成前体.本实验室从保存的犹他游动放线菌(Actinoplanes utahensis)SIPI-A.2001 基因组中克隆到ECB酰胺水解酶及其上下游基因,并将其构建入表达质粒pTGV2,通过接合转移的方法将此表达质粒导入到变铅青链霉菌(Streptomyc...

关 键 词:ECB酰胺水解酶  阿尼芬净  微生物转化

Construction of ECB deacylase genetically engineered strain and transformation of ECB
CHANG Qing,SHAO Lei,LI Ji-an,NI Bing,CHEN Dai-jie.Construction of ECB deacylase genetically engineered strain and transformation of ECB[J].Industrial Microbiology,2011,41(2):11-15.
Authors:CHANG Qing  SHAO Lei  LI Ji-an  NI Bing  CHEN Dai-jie
Institution:1. Life Science College; East China Normal University, Shanghai 200062 2. State Key Laboratory of New Drug and Pharmaceutical Process; Shanghai Institute of Pharmaceutical Industry, Shanghai 200040)
Abstract:Echinocandin B0 (ECB), a side-chain cleavaged nucleus, is a semi-synthetic precursor of significant antifungal anidulafungin. A fragment containing the ECB deacylase encoding region and its flank genes was amplified from Actinoplanes utahensis SIPI-A. 2001, and linked into the overexpression vector pTGV2. Then the plasmid was transformed into Streptornyces lizriclans TK24. This genetically engineered strain could transform ECB into ECB nucleus. HPLC analysis showed that the genetically engineered strain could effectively cleave ECB side chain, and the conversion rate was as high as 53.2%.
Keywords:ECB deacylase  anidulafungin  microbial conversion
本文献已被 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号