林可霉素生物合成培养基的优化

以花生粉和棉籽蛋白粉取代了原培养基中的黄豆饼粉,采用响应面法对林可霉素产生菌的发酵培养基进行了优化.首先通过单因素试验及正交实验确定替代氮源及其浓度,采用Plackett-Burman实验分析各因素的主效应,选出对响应值影响较大的3个因素,即花生粉、K2HPO4和玉米浆.对这些因素做爬坡实验,确定三个重要因素的中心点浓...

Optimization of biosynthesis medium for lincomycin production

The soybean flour was replaced by cottonseed protein and peanut meal as main nitrogen sources for lincomycin production. Single factor experiments and orthogonal test were carried out to determine the alternative nitrogen source and concentration. The response surface methodology was used to optimize the biosynthesis medium of Streptomyces linconinensis. First, peanut meal, KH2PO4 and corn steep liquor were found to be the important factors by Plackett-Burman design. Then center concentration of the three important factors was determined by the steepest ascent search. And the optimal concentration of the three important factors was defined by response surface methodology. The results were the following： 18 g/L peanut meal, 0.4 g/L KH2PO4 and 1.2 g/L corn steep liquor. The average potency of lincomycin could reach 4. 268 g/L by using the optimal medium, which was increased by 12% -17% compared with that of the original medium. Finally, metal ions and amino acid had good effects on lincomycin production by comprising differences between original culture and optimal one.