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| 紫外诱变原生质体选育D-核糖生产菌株 | |
| 引用本文： | 王慕华,孙文敬,郭金权,赵峰梅,秦丽,杨庆文.紫外诱变原生质体选育D-核糖生产菌株[J].工业微生物,2005,35(1):24-27. |
| 作者姓名： | 王慕华 孙文敬 郭金权 赵峰梅 秦丽 杨庆文 |
| 作者单位： | 1. 山西省生物研究所,太原,030006 2. 山西省生物研究所,太原,030006;河北师范大学生命科学学院,石家庄,050016 3. 郑州市生物化工厂,郑州,450001 |
| 基金项目： | 山西省科技攻关项目(NO.012055) |
| 摘 要： | 以D-核糖产生菌枯草芽孢杆(Bacillus subtilis)B941为出发菌株，采用紫外诱变原生质体的方法，获得了4株可以在含有6．0％D-核糖的培养基上生长的D-核糖高产菌株，其摇瓶发酵产糖达55．0g／L左右。通过摇瓶发酵试验，研究了D-核糖高产菌株Buvp-24的遗传稳定性。研究结果表明，经多次传代，菌株Buvp-24的发酵产糖能力及对发酵产物D-核糖的耐受性没有改变，有望应用于工业生产中。 |
| 关 键 词： | D-核糖 生产菌株 摇瓶发酵 高产菌株 紫外诱变 产生菌 原生质体 选育 遗传稳定性 发菌 |
Breeding of D-ribose producing mutants by protoplast mutagenesis of ultraviolet irradiation |
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| WANG Mu-hua,SUN Wen-jing,GUO Jin-quan,ZHAO Feng-mei,QIN Li,YANG Qing-wen.Breeding of D-ribose producing mutants by protoplast mutagenesis of ultraviolet irradiation[J].Industrial Microbiology,2005,35(1):24-27. | |
| Authors: | WANG Mu-hua SUN Wen-jing GUO Jin-quan ZHAO Feng-mei QIN Li YANG Qing-wen |
| Institution: | WANG Mu-hua1,SUN Wen-jing~1,2,GUO Jin-quan1,ZHAO Feng-mei1,QIN Li3,YANG Qing-wen1 |
| Abstract: | The D-ribose producing strain Bacillus subtilis B941 was selected as the parent strain. The protoplast of B. subtilis was mutagenized by ultraviolet irradiation, and four mutants with high D-ribose producing ability were obtained. The mutants could grow on the culture medium with 6.0% D-ribose, and produced about 55.0g/L D-ribose in the shaking flask. The hereditary stability of the mutant Buvp-24 was investigated in the shaking flask, and the results showed that the mutant D-ribose producing ability and D-ribose endurance had no change over 9 generations. The mutant may be applied in the industrial fermentation process. |
| Keywords: | D-ribose Bacillus subtilis protoplast fermentation |
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