| L-组氨酸产生菌的选育及发酵条件优化 |
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| 引用本文： | 侯颖,李江华,刘龙,堵国成,陈坚.L-组氨酸产生菌的选育及发酵条件优化[J].工业微生物,2013(6):18-25. |
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| 作者姓名： | 侯颖 李江华 刘龙 堵国成 陈坚 |
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| 作者单位： | [1]江南大学工业生物技术教育部重点实验室,无锡214122 [2]江南大学食品科学技术国家重点实验室,无锡214122 |
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| 基金项目： | 食品微生物的功能优化与调控的基础研究（973项目）（2012CB720802,2012CB720806）,江苏高校优势学科建设工程资助项目. |
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| 摘 要： | 以粘质沙雷氏菌（Serratiamarcescens）ATCC31026为出发菌株，经过硫酸二乙酯（DES）、亚硝基胍（NTG）和紫外（uV）逐级诱变，选育出了一株具有3-氨基-1，2，4-三氮唑抗性（AMTr）、6-巯基嘌呤抗性（6．MPr）、组氨酸甲酯抗性（HEr）、2-硫尿嘧啶抗性（2-Tur）、D-组氨酸抗性（DHr）等遗传标记的突变株ZJZ625（AMTr6．MPrHEr2-TUrDHr），L-组氨酸产量由最初的2．0g／L提高到7．6g/L。对ZJZ625进行培养基及培养条件优化，研究了单因素对发酵的影响，由响应面分析得出最佳培养基配方，使最终产量达到9．7g／L。
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| 关 键 词： | L-组氨酸 粘质沙雷氏菌 选育 优化 响应面 |
Breeding and fermentation condition optimization of L-histidine producing strain |
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| HOU Ying,LI Jiang-hua,LIU Long,DU Guo-cheng,CHEN Jian.Breeding and fermentation condition optimization of L-histidine producing strain[J].Industrial Microbiology,2013(6):18-25. |
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| Authors: | HOU Ying LI Jiang-hua LIU Long DU Guo-cheng CHEN Jian |
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| Institution: | 1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; 2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China) |
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| Abstract: | The Serratia marcescens ATCC 31026 was used as the starting strain. By stepwise mutagenic treatments with N- methyl-N-nitro-N-nitrosoguanidine（NTG）, diethylsulfate （ DES ） and ultraviolet （ UV ）, the objective mutant ZJZ 625 （ AMTr6-MPrHEr2-TUr6-MEPrDHr） was obtained. The mutants was selected on the agar plates containing 3-amino-1,2,4 triazole, 6-mercaptopurine arabinoside, L-histidine methyl ester dihydrochloride, 2-thiouracil and D-histidine. The L-his- tidine producing mutant ZJZ 625 could accumulate 7.6 g/L. Based on the effects of single factors and then the response surface analysis was used to optimize the concentrations of the substrate. The amount of L-histidine accumulation reached to a level of 9.7 g/L. |
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| Keywords: | L-histidine Serratia marcescens breeding optimization response surface analysis |
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