| 葡萄糖流加策略对基因工程FR-008／杀念菌素衍生物CS103补料分批发酵过程的影响 |
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| 引用本文： | 毛相朝,杨亮,陈实,沈亚领,魏东芝,邓子新.葡萄糖流加策略对基因工程FR-008／杀念菌素衍生物CS103补料分批发酵过程的影响[J].工业微生物,2009,39(2):1-6. |
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| 作者姓名： | 毛相朝 杨亮 陈实 沈亚领 魏东芝 邓子新 |
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| 作者单位： | 1. 华东理工大学生物反应器工程国家重点实验室,鲁华生物技术研究所,上海,200237
2. 上海交通大学微生物代谢教育部重点实验室,上海,200030 |
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| 基金项目： | 上海市创新行动计划重大项目 |
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| 摘 要： | 以组合生物合成技术得到的链霉菌FR-008突变株CS103为研究对象，研究了3．7L发酵罐上维持一定葡萄糖浓度对其次级代谢产物脱羧FR-008／candicidin衍生聚酮抗生素CS103生物合成的影响。当初始葡萄糖浓度20g／L，发酵过程还原糖浓度维持在10g/L时，抗生素CS103最高产量较分批发酵最高产量相比提高30％。研究了3．7L罐上补料分批发酵生产CS103的工艺，主要考察了脉冲补料、间歇流加补料和连续流加补料三种补料分批发酵工艺，并与分批发酵进行了比较。连续流加补料维持糖浓度的效果明显，最高产量达到126．9μg/mL，与分批发酵相比提高了44％左右。
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| 关 键 词： | 葡萄糖 流加策略 补料分批发酵 基因工程FR-008/杀念菌素 |
Influence of glucose feeding strategy on fed-batch cultures of a novel FR-008/candicidin derivatives CS103 by a mutant of Streptomyces sp. FR-008 |
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| MAO Xiang-Zhao,YANG Liang,CHEN Shi,SHEN Ya-Ling,WEI Dong-Zhi,DENG Zi-Xin.Influence of glucose feeding strategy on fed-batch cultures of a novel FR-008/candicidin derivatives CS103 by a mutant of Streptomyces sp. FR-008[J].Industrial Microbiology,2009,39(2):1-6. |
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| Authors: | MAO Xiang-Zhao YANG Liang CHEN Shi SHEN Ya-Ling WEI Dong-Zhi DENG Zi-Xin |
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| Institution: | MAO Xiang-Zhao, YANG Liang, CHEN Shi, SHEN Ya-Ling , WEI Dong-Zhi , DENG Zi-Xin(1.State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237; 2. Laboratory of Microbial Metabolism and School of Life Science ＆ Biotechnology, Shanghai Jiaotong University, Shanghai 200030) |
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| Abstract: | The mutant of Streptomyces sp. FR-008 was conducted through biochemistry method, and its products could be the novel derivatives of polyene macrolides antibiotic FR-008/eanclicidin. The maintained glucose concentration and the corresponding production of the novel antibiotic CS103 were investigated in the 3.7 L fermentor. The optimum substrate of glucose concentration （10 g/L） was obtained for maximal antibiotic CS103 production, increased about 30 96 compared with the batch culture. In fed-batch experiments, pulse-feeding mode, intermittent feeding mode and continuous-feeding mode were compared. The continuous-feeding was observed to be the best strategy than other two feeding modes, in terms of antibiotic yields. The production could reach 126.9 μg/mL, increased about 44% compared with that of the batch culture. |
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| Keywords: | glucose feeding strategy fed-batch fermentation novel FR-008/candicidin derivatives |
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