| 真空微波诱变结合化学诱变选育酸性纤维素酶高产菌株 |
| |
| 引用本文： | 王瑾,邬敏辰,张树飞.真空微波诱变结合化学诱变选育酸性纤维素酶高产菌株[J].工业微生物,2009,39(4):27-31. |
| |
| 作者姓名： | 王瑾 邬敏辰 张树飞 |
| |
| 作者单位： | 1. 江南大学生物工程学院江南大学生物技术教育部重点实验室
2. 江南大学医药学院,无锡,214122 |
| |
| 摘 要： | 以酸性纤维素酶产生菌绿色木霉（Trichoderma viride）WL0512作为原始出发菌株，首先经自然分离筛选出一株产酶较稳定的菌株TVN-18，其羧甲基纤维素酶活（CMC酶活）达2765．8U／g，滤纸酶活（FPA酶活）达48．5U／g。再经真空微波和甲基磺酸乙酯（EMS）逐级诱变处理，获得了一株高产、稳产酸性纤维素酶的E6—1菌株，其CMC酶活达4396．6U／g，FPA酶活达126．0U／g，分别是菌株TVN-18的1．59倍和2．60倍。通过对固态发酵培养基麸皮和稻草比例、料水比以及初始pH值的优化，突变株的产酶能力进一步得到提高，其产的CIVIC酶活和FPA酶活分别提高了22．3％和22．4％。
|
| 关 键 词： | 纤维素酶 绿色木霉 真空微波辐照 EMS处理 固态发酵 |
Breeding of high-yield cellulase mutant strains with vacuum microwave irradiation and ethyl methane-sulfonate |
| |
| WANG Jin,WU Min-chen,ZHANG Shu-fei.Breeding of high-yield cellulase mutant strains with vacuum microwave irradiation and ethyl methane-sulfonate[J].Industrial Microbiology,2009,39(4):27-31. |
| |
| Authors: | WANG Jin WU Min-chen ZHANG Shu-fei |
| |
| Institution: | WANG Jin,WU Min-chen, ZHANG Shu-fei(1. School of Biotechnology; 2. Key Laboratory of Industury Biotechnology Under Ministry of Education 3. School of Medicine and Pharrnaeeutics, Jiangnan University, Wuxi 214122, China) |
| |
| Abstract: | A high yield cellulase producing strain Trichoderma viride TVN-18 was isolated by natural separation. After treated with vacuum microwave irradiation and ethyl methane-sulfonate, a higher yield acidic cellulase producing strain Trichoderma viride E6-1 was obtained. The CMCase was increased by 59% from 2765.8 U/g to 4396.6 U/g, and the FPase was increased by 160% from 48.5 U/g to 126.0 U/g. Its solid state fermentation (SSF) was performed by using different initial pH, ratios of rice straw to wheat bran and material to water. The CMCase and FPase of the strain E6-1 growing on the optimal SSF were increased by 22.3% and 22.4%, respectively. |
| |
| Keywords: | cellulose Trichoderma virider vacuum microwave irradiation EMS treatment solid state fermentation |
| 本文献已被 维普 万方数据 等数据库收录！ |
