基于核基因RAG2部分序列探索菊头蝠总科、蝙蝠科和鞘尾蝠科物种的系统发育关系

采用PCR技术获得了翼手目(菊头蝠总科、蝙蝠科以及鞘尾蝠科)16种19个个体的核基因重组激活基因2(Recombination activating gene,RAG2)部分序列,长度为730～760 bp.结合从Genbank中提取的翼手目3科14个体的RAG2序列,通过构建贝叶斯(Bayesian inference,BI)和最大似然树对翼手目种属间的系统进化关系进行研究.研究表明:在菊头蝠总科中,菊头蝠科和蹄蝠科是两个独立的科,且在蹄蝠科中,大蹄蝠、中蹄蝠、普氏蹄蝠3种之间的亲缘关系非常近;蝙蝠科中的南蝠属与棕蝠属是姊妹群;长翼蝠亚科应提升为长翼蝠科.此外,鞘尾蝠科与犬吻蝠科形成姊妹群关系.

Phylogenetics of Rhinolophidea, Vespertilionidae and Emballonuridae Based on Partial Sequences of the Nuclear RAG2 Gene

The partial sequences of nuclear RAG2 gene(Recombination activating gene,RAG2)were ob-tained from 19 individuals of 16 species of Chiroptera(Rhinolophidea,Vespertilionidae and Emballonuridae)from Guizhou Province.The lengths of RAG2 are 730～760 bp.The evolutionary trees was produced by Bayesian inference(BI) and Maximum likelihood(ML) methods,combined with the sequences RAG2 of 14 individuals of 3 families from Genbank,to study the relationship of species or genera of Chiroptera.It suggested that: Rhinolophidae and Hipposideridae were two separate families and H.pratti,H.armiger and H.larvatus have more closely genetic relationship in Hipposideridae,Ia genus and Eptesicus genus was sister group in Vespertilionidae.The subfamily Miniopterinae should be elevated to family status.In addition,Emballonuri-dae and Molossidae form sister group.