南蝠线粒体DNA控制区结构及贵州7个种群遗传多样性的分析研究

采用PCR技术获得了贵州7个南蝠(Ia io)自然种群42个个体的线粒体DNA控制区全序列,长度为1256～1340 bp.对控制区结构进行分析,识别了其延伸的终止结合序列区(包括ETAS1和ETAS2元件)、中央保守区(包括F、E、D、C、B元件)和保守序列区(包括CSB1、CSB2和CSB3元件);同时,在延伸的终止结合序列区还发现了若干能形成发夹结构的主体序列TACAT—ATGTA.在7个自然种群42个个体中共定义了16个单倍型.遗传多样性分析表明:贵州南蝠种群具有较高的单倍型多样性(h=0.945)和中等的核苷酸多样性(π=0.012).基因流、AMOVA和系统进化树分析表明贵州这7个南蝠自然群体间没有发生遗传分化.

Structure of the Mitochondrial DNA Control Region and Genetic Diversity Analysis of Seven la io Populations in Guizhou

The sequences of the control region(D-loop) of mitochondrial DNA(mtDNA) were obtained from 42 individuals of seven I.io populations from Guizhou province.The lengths of D-loop region are 1 256～1 340 bp.It could be divided into three domains,the extended termination associated sequence(including ETAS1and ETAS2),the central conserved sequence domain(including F,E,D,C,B boxes) and the conserved sequence block domain(including CSB1,CSB2,CSB3).And a sequence(TACAT) and its reverse complementary se-quence(ATGTA) were found in the ETAS domain.16 haplotypes were identified from 42 individuals of 7 I.io populations.I.io populations from Guizhou have high haplotype diversity(h=0.945) and medium nu-cleotide diversity(π=0.012).Gene flow,AMOVA and phylogenetic tree show that there is no genetic differ-entiation among those populations.