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| 人胚胎干细胞在3种培养体系中的生长 | |
| 引用本文： | 许慧芳,陈红,焦淑洁,黎逢光,张苏明.人胚胎干细胞在3种培养体系中的生长[J].生命科学研究,2009,13(1). |
| 作者姓名： | 许慧芳 陈红 焦淑洁 黎逢光 张苏明 |
| 作者单位： | 1. 华中科技大学同济医学院附属同济医院神经内科,中国,湖北,武汉,430030 2. 华中科技大学同济医学院附属同济医院康复科,中国,湖北,武汉,430030 3. 中国人民解放军161医院神经内科,中国,湖北,武汉,430000 |
| 摘 要： | 人胚胎干细胞(human embryonic stem cells.hESCs)的培养一直是干细胞研究的重要内容.用本实验室独立建系的两株hESCs,建立3种不同的培养体系:小鼠胚胎成纤维细胞(mouse embryonic fibroblasts,MEFs)做饲养层,永生化人成纤维细胞(immortalized human adult fibroblasts,HAFi)做饲养层,无饲养层条件培养基培养体系(condition medium,CM),观察在3种培养体系中,干细胞的增殖和分化情况.发现3种培养体系中的hESCs都可以表达一致的生物学特性,但也有不同之处,相对于CM干细胞在MEFs和HAFi饲养层体系的分化率低,增殖快;但MEFs来源于鼠类是异源细胞,HAFi虽不舍鼠源性成分却繁殖很慢;无饲养层的体系便于操作,无外源细胞存在.实验所得出的结果可以引导研究人员针对于临床、科研不同的需要,选择最适合的培养体系. |
| 关 键 词： | 人胚胎干细胞 培养体系 无饲养层培养 |
Growth of Human Embryonic Stem Cells in Three Culture Systems |
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| XU Hui-fang,CHEN Hong,JIAO Shu-jie,LI Feng-guang,ZHANG Su-ming.Growth of Human Embryonic Stem Cells in Three Culture Systems[J].Life Science Research,2009,13(1). | |
| Authors: | XU Hui-fang CHEN Hong JIAO Shu-jie LI Feng-guang ZHANG Su-ming |
| Institution: | XU Hui-fang1,CHEN Hong2,JIAO Shu-jie1,LI Feng-guang3,ZHANG Su-ming1 (1. Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei,China,2. Department of Rehabilitation,3. Department of Neurology,the 161st Center Hospital of People's Liberation Army,Wuhan 430010,China) |
| Abstract: | Culture systems for human embryonic stem cells (hESCs)were important for hESCs researches. Three different culture systems were used as follows:mouse embryonic fibroblasts (MEFs)as feeder-layer, immortalized human adult fibroblasts (HAFi)as feeder-layer and feeder-free system with condition medium (CM). The two cell lines were established in our laboratory. Information of growth and differentiation of hESCs were detected. It was found that both lines could maintain all their characteristics in these three c... |
| Keywords: | human embryonic stem cell culture system feeder-free culture |
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