| 斑马鱼心脏发育候选基因Pygo1多克隆抗体的制备及检测 |
| |
| 引用本文： | 唐雄卓,江志钢,赵碧峰,李发祥,谢华平,邓云,吴秀山.斑马鱼心脏发育候选基因Pygo1多克隆抗体的制备及检测[J].生命科学研究,2011,15(4):294-297. |
| |
| 作者姓名： | 唐雄卓 江志钢 赵碧峰 李发祥 谢华平 邓云 吴秀山 |
| |
| 作者单位： | 湖南师范大学蛋白质化学及鱼类发育生物学教育部重点实验室,心脏发育研究中心,中国湖南长沙410081 |
| |
| 基金项目： | 国家自然科学基金资助项目(30930054) |
| |
| 摘 要： | Pygo1是心脏发育候选基因,可能在斑马鱼心脏发育过程中起着重要的调控作用.利用生物信息学选择斑马鱼Pygo1基因抗原亲水区,将扩增出的PCR片段克隆到原核表达pGEMT-4T-1载体中,转入E.coli中后通过IPTG(Isopropylβ-D-thiogalactoside)分别诱导表达GST融合蛋白.蛋白经纯化分离后用于免疫新西兰大白兔制备多克隆抗体.用Western Blot检测抗体的效价和特异性.
|
| 关 键 词： | Pygo1基因 斑马鱼 原核表达 多克隆抗体 |
Preparation and Detection of Polyclonal Antibody of Heart Development Candidate Genes Pygo1 in Zebrafish |
| |
| TANG Xiong-zhuo,JIANG Zhi-gang,ZHAO Bi-feng,LI Fa-xiang,XIE Hua-ping,DENG Yun,WU Xiu-shan.Preparation and Detection of Polyclonal Antibody of Heart Development Candidate Genes Pygo1 in Zebrafish[J].Life Science Research,2011,15(4):294-297. |
| |
| Authors: | TANG Xiong-zhuo JIANG Zhi-gang ZHAO Bi-feng LI Fa-xiang XIE Hua-ping DENG Yun WU Xiu-shan |
| |
| Institution: | TANG Xiong-zhuo,JIANG Zhi-gang,ZHAO Bi-feng,LI Fa-xiang,XIE Hua-ping,DENG Yun,WU Xiu-shan*(The Center for Heart Development,Key Lab of MOE for Development Biology and Protein Chemistry,Hunan Normal University,Changsha 410081,Hunan,China) |
| |
| Abstract: | Pygo1 is a candidate gene for heart development,which might play an important role in Zebrafish heart development.Hydrophilic antigen region of Pygo1 is selected by bioinformatic method.Pygo1 fragment was amplified by PCR and purified.The fragment was inserted into the prokaryotic expression vector pGEMT-4T-1.This new recombined plasmid was transformed into E.coli and expression of the GST fusion protein was induced with IPTG.After purified by GST affinity chromatography,GST-Pygo1 fusion protein was used to... |
| |
| Keywords: | Pygo1 Zebrafish prokaryotic expression polyclonal antibody |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
