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| 靶向抑制miRNA-21提高白血病K562细胞对阿糖胞苷的敏感性 | |
| 引用本文： | 朱雪姣,李育敏,谷景义,郭敏,费嘉.靶向抑制miRNA-21提高白血病K562细胞对阿糖胞苷的敏感性[J].生命科学研究,2011,15(4). |
| 作者姓名： | 朱雪姣 李育敏 谷景义 郭敏 费嘉 |
| 作者单位： | 1. 暨南大学医学院生物化学与分子生物学教研室,中国广东广州,510632 2. 广西壮族自治区民族医院,中国广西南宁,530001 3. 暨南大学医学院血液病研究所,中国广东广州,510632 |
| 基金项目： | 暨南大学科研培育与创新基金前瞻性与基础研究项目(11609406),暨南大学研究生精品课程(医学分子生物学)建设项目(50623021) |
| 摘 要： | 为研究以miRNA-21为靶标的反义核酸(AMO-miR-21)提高白血病K562细胞对阿糖胞苷(Ara-C)的敏感性及可能的作用机制,在LipofectamineTM2000介导下,将化学合成的AMO-miR-21转染K562细胞,四甲基偶氮唑蓝(MTT)法检测单独使用AMO-miR-21、Ara-C以及两者联合使用对细胞增殖抑制作用,并计算抑制率和IC50;流式细胞仪检测细胞凋亡;实时定量PCR(Real-time PCR)检测miRNA-21及其候选靶基因Pdcd4 mRNA的表达水平;免疫印迹(Western blot)检测Pdcd4蛋白的表达水平.结果显示Ara-C与AMO-miR-21联合使用后,IC50从1.95μmol/L降低到0.84μmol/L,敏感性提高到单用Ara-C的2.3倍.AMO-miR-21联合Ara-C组细胞凋亡明显增加(P<0.05).AMO-miR-21显著抑制K562细胞中miRNA-21的表达水平(P<0.05),同时Pdcd4的表达明显增加(P<0.05).提示AMO-miR-21可以提高K562细胞对Ara-C的敏感性,其作用机制与靶向抑制miRNA-21,进而诱... |
| 关 键 词： | miRNA-21 反义寡核苷酸 阿糖胞苷 K562细胞 化疗敏感性 |
Enhanced Chemosensitivty of Leukemic Cells to Cytarabine by Targeted Suppression of miRNA-21 |
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| ZHU Xue-jiao,LI Yu-min,GU Jing-yi,GUO Min,FEI Jia.Enhanced Chemosensitivty of Leukemic Cells to Cytarabine by Targeted Suppression of miRNA-21[J].Life Science Research,2011,15(4). | |
| Authors: | ZHU Xue-jiao LI Yu-min GU Jing-yi GUO Min FEI Jia |
| Institution: | ZHU Xue-jiao1,LI Yu-min2,GU Jing-yi1,GUO Min3,FEI Jia1*(1.Department of Biochemistry and Molecular Biology,Medical College of Jinan University,Guangzhou 510632,Guangdong,China,2.Minzu Hospital of Guangxi Zhuang Autonomous Region,Nanning 530001,Guangxi,3.Institute of Hematology,China) |
| Abstract: | Our goal is to investigate the influences of anti-miRNA-21 oligonucleotide(AMO-miR-21) on Ara-C chemosensitivity in human leukemic K562 cells,which were incubated with different concentration of Ara-C alone or in combination with AMO-miR-21.The growth-inhibitory potencies were measured by MTT assay.The inhibition rate and IC50 was calculated.The cell apoptosis was assessed by flow cytometry.The expres-sion of miRNA-21 and its potential target gene Pdcd4 was detected by real-time PCR and Western blot in K562... |
| Keywords: | miRNA-21 antisense oligonucleotide Ara-C leukemia K562 cell chemosensitivity |
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